Expression of growth-related genes in young and older human skeletal muscle following an acute stimulation of protein synthesis
Expression of growth-related genes in young and older human skeletal muscle following an acute stimulation of protein synthesis
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DOI：
10.1152/japplphysiol.90842.2008
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发表时间：
2009-04-01
影响因子：
3.3
通讯作者：
Rasmussen, Blake B.
中科院分区：
文献类型：
--
作者：
Drummond, Micah J.;Miyazaki, Mitsunori;Rasmussen, Blake B.
Drummond MJ，宫崎M，Dreyer HC，Pennings B，Dhanani S，Volpi E，Esser KA，Rasmussen BB.急性刺激蛋白质合成后年轻和老年人骨骼肌中生长相关基因的表达。J Appl Physiol 106：1403 - 1411，2009.首次发表于2008年9月11日; doi：10.1152/japplphysiol.90842.2008。肌肉生长与mTOR信号通路和卫星细胞调节因子的激活有关。本研究的目的是确定17个与mTOR/肌肉蛋白合成和卫星细胞/生肌程序相关的选定基因是否在年轻人和老年人骨骼肌中在休息时和对有效的合成代谢刺激[抗阻运动+必需氨基酸摄入（RE + EAA）]的反应中差异表达。12名男性受试者（6名年轻人，6名老年人）完成了一次大阻力运动。在RE + EAA之前和之后3小时和6小时获得肌肉活检。受试者在运动后1小时摄入富含亮氨酸的必需氨基酸。使用qRT-PCR测定mRNA表达。在静息状态下，hVps34 mRNA在老年受试者中升高（P <0.05），而myoD、肌细胞生成素和TSC 2 mRNA的水平有高于年轻受试者的趋势。合成代谢刺激（RE + EAA）改变了与mTOR调节相关的mRNA。REDD2在两个年龄组均显著降低（P <0.05），而Rheb mRNA表达仅在青年组升高。RE + EAA后6h，青年组和老年组cMyc mRNA均显著升高（P <0.05）。此外，RE + EAA还增加了年轻人中与卫星功能相关的几种mRNA的表达（P <0.05），而老年人中这些mRNA的表达没有变化。我们的结论是，肌肉中的几个合成代谢基因在RE + EAA后的年轻男性中反应更灵敏。我们的数据为RE + EAA后年轻和老年人骨骼肌中对转录和翻译重要的基因调控提供了新的见解。
Drummond MJ, Miyazaki M, Dreyer HC, Pennings B, Dhanani S, Volpi E, Esser KA, Rasmussen BB. Expression of growth-related genes in young and older human skeletal muscle following an acute stimulation of protein synthesis. J Appl Physiol 106: 1403-1411, 2009. First published September 11, 2008; doi:10.1152/japplphysiol.90842.2008.-Muscle growth is associated with an activation of the mTOR signaling pathway and satellite cell regulators. The purpose of this study was to determine whether 17 selected genes associated with mTOR/muscle protein synthesis and the satellite cells/myogenic program are differentially expressed in young and older human skeletal muscle at rest and in response to a potent anabolic stimulus [resistance exercise + essential amino acid ingestion (RE+EAA)]. Twelve male subjects (6 young, 6 old) completed a bout of heavy resistance exercise. Muscle biopsies were obtained before and at 3 and 6 h post RE+EAA. Subjects ingested leucine-enriched essential amino acids at 1 h post-exercise. mRNA expression was determined using qRT-PCR. At rest, hVps34 mRNA was elevated in the older subjects (P < 0.05) while there was a tendency for levels of myoD, myogenin, and TSC2 mRNA to be higher than young. The anabolic stimulus (RE+EAA) altered mRNAs associated with mTOR regulation. Notably, REDD2 decreased in both age groups (P < 0.05) but the expression of Rheb mRNA increased only in the young. Finally, cMyc mRNA was elevated (P < 0.05) in both young and old at 6 h post RE+EAA. Furthermore, RE+EAA also increased expression of several mRNAs associated with satellite function in the young (P < 0.05), while expression of these mRNAs did not change in the old. We conclude that several anabolic genes in muscle are more responsive in young men post RE+EAA. Our data provide new insights into the regulation of genes important for transcription and translation in young and old human skeletal muscle post RE+EAA.