Functional differences between keratins of stratified and simple epithelia.
Functional differences between keratins of stratified and simple epithelia.
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DOI：
10.1083/jcb.143.2.487
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发表时间：
1998-10-19
影响因子：
7.8
通讯作者：
Fuchs, E
中科院分区：
文献类型：
--
作者：
Hutton, E;Paladini, RD;Yu, QC;Yen, M;Coulombe, PA;Fuchs, E
分化的层状和单层上皮组织分别表达角蛋白5和14，角蛋白8和18。有人认为，这些角蛋白形成了一个对细胞完整性很重要的机械框架，因为它们的缺失会导致新生儿表皮的起泡皮肤紊乱，并导致胚胎肝脏内的出血。一个尚未解决的基本问题是，不同的角蛋白是否在上皮细胞中发挥独特的功能。我们现在使用转基因技术来解决这个问题，在K14缺失的小鼠的表皮中表达K16-14杂交表皮角蛋白转基因和K18简单上皮角蛋白转基因。在杂交表皮角蛋白将野生型表型恢复到新生表皮的条件下，K18部分但不是完全挽救。解释似乎并不在于K18不能与K5在体外和体内形成10纳米的细丝。相反，K5和K18之间形成的角蛋白网络似乎缺乏抵抗机械应力的能力，导致皮肤自然或机械损伤区域的角蛋白网络受到干扰。综上所述，这些发现表明，I型表皮角蛋白的丢失不能被其对应的简单上皮细胞完全补偿，而且在体内，所有角蛋白并不是等同的。
Dividing populations of stratified and simple epithelial tissues express keratins 5 and 14, and keratins 8 and 18, respectively. It has been suggested that these keratins form a mechanical framework important to cellular integrity, since their absence gives rise to a blistering skin disorder in neonatal epidermis, and hemorrhaging within the embryonic liver. An unresolved fundamental issue is whether different keratins perform unique functions in epithelia. We now address this question using transgenic technology to express a K16-14 hybrid epidermal keratin transgene and a K18 simple epithelial keratin transgene in the epidermis of mice null for K14. Under conditions where the hybrid epidermal keratin restored a wild-type phenotype to newborn epidermis, K18 partially but not fully rescued. The explanation does not appear to reside in an inability of K18 to form 10-nm filaments with K5, which it does in vitro and in vivo. Rather, it appears that the keratin network formed between K5 and K18 is deficient in withstanding mechanical stress, leading to perturbations in the keratin network in regions of the skin that are subjected either to natural or to mechanically induced trauma. Taken together, these findings suggest that the loss of a type I epidermal keratin cannot be fully compensated by its counterpart of simple epithelial cells, and that in vivo, all keratins are not equivalent.
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影响因子：
7.8
作者：
Kowalczyk, AP;Bornslaeger, EA;Borgwardt, JE;Palka, HL;Dhaliwal, AS;Corcoran, CM;Denning, MF;Green, KJ
通讯作者：
Green, KJ
DOI：
10.1083/jcb.127.1.161
发表时间：
1994-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ku NO;Omary MB
通讯作者：
Omary MB
DOI：
10.1083/jcb.110.4.1199
发表时间：
1990-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hatzfeld M;Weber K
通讯作者：
Weber K
影响因子：
10.5
作者：
CHAN, YM;ANTONLAMPRECHT, I;FUCHS, E
通讯作者：
FUCHS, E
影响因子：
64.8
作者：
LANE, EB;RUGG, EL;EADY, RAJ
通讯作者：
EADY, RAJ