The analysis of core and symbiotic genes of rhizobia nodulating Vicia from different continents reveals their common phylogenetic origin and suggests the distribution of Rhizobium leguminosarum strains together with Vicia seeds
The analysis of core and symbiotic genes of rhizobia nodulating Vicia from different continents reveals their common phylogenetic origin and suggests the distribution of Rhizobium leguminosarum strains together with Vicia seeds
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DOI：
10.1007/s00203-009-0495-6
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发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Velazquez, Encarna
中科院分区：
文献类型：
--
作者：
Alvarez-Martinez, Estela R.;Valverde, Angel;Velazquez, Encarna
本文分析了从西班牙西北部三种土壤中分离到的油菜根瘤菌的核心基因和共生基因，并与其他地区分离到的油菜内共生菌进行了比较。rrs、recA和atpD基因及16S-23S rRNA基因间隔分析表明，西班牙根瘤菌与欧洲、美洲和亚洲不同国家的苜蓿根瘤菌和蚕豆根瘤菌分离株在系统发育上接近，形成了一个与豆科根瘤菌相关的类群。对不同大陆结瘤株sativa和faba的nodC基因分析表明，它们属于一个系统发育紧密的类群，表明这两种豆科植物是限制性寄主。nodC基因分析结果表明，油菜和蚕豆内共生体在几个大陆具有共同的系统发育起源。由于这两种豆科植物都来自欧洲，因此我们的研究结果表明，在这种根瘤菌-蚕豆共生中，豆科植物的菌株与sativa和faba种子一起分布在世界各地，并且在染色体、共生基因和豆科植物宿主之间存在密切的共同进化。
In this work, we analysed the core and symbiotic genes of rhizobial strains isolated from Vicia sativa in three soils from the Northwest of Spain, and compared them with other Vicia endosymbionts isolated in other geographical locations. The analysis of rrs, recA and atpD genes and 16S-23S rRNA intergenic spacer showed that the Spanish strains nodulating V. sativa are phylogenetically close to those isolated from V. sativa and V. faba in different European, American and Asian countries forming a group related to Rhizobium leguminosarum. The analysis of the nodC gene of strains nodulating V. sativa and V. faba in different continents showed they belong to a phylogenetically compact group indicating that these legumes are restrictive hosts. The results of the nodC gene analysis allow the delineation of the biovar viciae showing a common phylogenetic origin of V. sativa and V. faba endosymbionts in several continents. Since these two legume species are indigenous from Europe, our results suggest a world distribution of strains from R. leguminosarum together with the V. sativa and V. faba seeds and a close coevolution among chromosome, symbiotic genes and legume host in this Rhizobium-Vicia symbiosis.