Induction of immunoglobulin G1, interleukin-6 and interleukin-10 by Taenia crassiceps metacestode carbohydrates
Induction of immunoglobulin G1, interleukin-6 and interleukin-10 by Taenia crassiceps metacestode carbohydrates
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DOI：
10.1046/j.1365-2567.2002.01519.x
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发表时间：
2002-12-01
期刊：
影响因子：
6.4
通讯作者：
Lukic, M
中科院分区：
文献类型：
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作者：
Dissanayake, S;Khan, N;Lukic, M
辅助性T细胞2型(Th2)极化免疫反应在蠕虫感染中占主导地位。两个显示Th1到Th2极化随着感染进展的小鼠模型是曼氏血吸虫和粗隆带绦虫。在这两种情况下，早期的Th1反应被晚期的Th2反应所取代。我们报道了粗圆头肌中的核酸、蛋白质和无脂碳水化合物部分(简称T-CHO)具有Th2样免疫调节活性。在T-CHO存在下，用鸡白蛋白免疫两个品系的大鼠(Dark Agti和Albino Oxford)和BALB/c小鼠，可选择性地增强免疫球蛋白G1(IgG1)抗体，该抗体被认为与大鼠和小鼠的Th2反应有关。在T-CHO刺激的小鼠脾细胞体外培养中，IL-6和IL-10是主要的细胞因子。培养上清液中未检测到IL-4和IL-5。此外，带绦虫碳水化合物对脾细胞具有促有丝分裂作用，能激活蛋白质的丝氨酸磷酸化，并上调抗凋亡蛋白Bcl2的表达。当小鼠脾细胞在含带绦虫碳水化合物的条件下培养时，IL-2的表达呈浓度依赖性下调，而IL-6的分泌呈重叠的上调。
T helper type 2 (Th2)-polarized immune responses are characteristically dominant in helminth infections. Two murine models that show a Th1 to Th2 polarization with infection progression are those of Schistosoma mansoni and Taenia crassiceps. In both, an early Th1 response is replaced by a late Th2 response. We report that the nucleic acid-, protein- and lipid-free carbohydrate fraction of T crassiceps metacestodes (denoted T-CHO) possesses Th2-like immunodulatory activity. Immunization of two strains of rats (Dark Agouti and Albino Oxford) and BALB/c mice with chicken albumin in the presence of T-CHO resulted in selective enhancement of immunoglobulin G1 (IgG1) antibodies, considered to be associated with Th2 responses in both rats and mice. Interleukin-6 (IL-6) followed by IL-10 were the dominant cytokines detected in in vitro cultures of mouse spleen cells stimulated with T-CHO. IL-4 and IL-5 were not detected in these culture supernates. Furthermore, Taenia carbohydrates were mitogenic to spleen cells, activated serine phosphorylation of proteins and up-regulated the expression of the anti-apoptotic protein, Bcl-2. When mouse spleen cells were cultured in the presence of Taenia carbohydrates, a concentration-dependent down-regulation of IL-2 and an overlapping up-regulation of IL-6 secretion were seen.