Combined biochemical and morphological study of particulate fractions from rat liver. Analysis of preparations enriched in lysosomes or in particles containing urate oxidase, D-amino acid oxidase, and catalase.
Combined biochemical and morphological study of particulate fractions from rat liver. Analysis of preparations enriched in lysosomes or in particles containing urate oxidase, D-amino acid oxidase, and catalase.
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DOI：
10.1083/jcb.26.1.219
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发表时间：
1965-07
影响因子：
7.8
通讯作者：
De Duve, C
中科院分区：
文献类型：
--
作者：
Baudhuin, P;Beaufay, H;De Duve, C
在不同的实验条件下，从大鼠肝脏中分离的六种颗粒制剂进行了生化分析和电子显微镜检查。结果证实了小管周围致密体的溶酶体性质，并表明微体是尿酸氧化酶、过氧化氢酶和D-氨基酸氧化酶的载体。过氧化氢酶，代表颗粒的主要成分，和D-氨基酸氧化酶似乎与无结构的“汁液”的颗粒，尿酸氧化酶与它们的晶体核心或与它们的外膜。
Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate oxidase, catalase, and D-amino acid oxidase. Catalase, representing a major component of the particles, and D-amino acid oxidase appear to be associated with the structureless "sap" of the particles, urate oxidase with their crystalloid core or with their outer membrane.