The role of miR-2∼13∼71 cluster in resistance to deltamethrin in Culex pipiens pallens
The role of miR-2∼13∼71 cluster in resistance to deltamethrin in Culex pipiens pallens
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DOI：
10.1016/j.ibmb.2017.03.006
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发表时间：
2017-05-01
影响因子：
3.8
通讯作者：
Zhu, Changliang
中科院分区：
文献类型：
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作者：
Guo, Qin;Huang, Yun;Zhu, Changliang
过量、持续使用溴氰菊酯导致蚊子对溴氰菊酯产生抗药性，成为蚊虫控制的主要障碍。在先前的研究中，使用Illumina测序法鉴定了淡色库蚊花粉中溴氰菊酯敏感（DS）品系和溴氰菊酯抗性（DR）品系之间差异表达的miRNA。本研究应用RNAi技术和美国疾病控制和预防中心（CDC）的瓶内生物测定法，探讨miR-2 similar to 13 similar to 71 cluster（miR-2、miR-13和miR-71）与溴氰菊酯抗性的关系。我们使用定量实时PCR（qRT-PCR）来测量类似于13个类似于71个簇的miR-2的表达水平。miR-2 similar to 13 similar to 71 cluster在DR品系雌蚊体内表达下调，并通过调节靶基因CYP 9 J35和CYP 325 BG 3在溴氰菊酯抗性中发挥重要作用。敲低CYP 9 J35和CYP 325 BG 3导致DR蚊的死亡率降低。这项研究提供了第一个证据表明，miRNA簇与蚊子的溴氰菊酯抗性有关。此外，我们还研究了miR-2 similar to 13 similar to 71 cluster与其靶基因之间形成的调控网络，为深入了解溴氰菊酯抗性机制提供了新的思路。(C)2017爱思唯尔有限公司版权所有
Excessive and continuous application of deltamethrin has resulted in the development of deltamethrin resistance among mosquitoes, which becomes a major obstacle for mosquito control. In a previous study, differentially expressed miRNAs between deltamethrin-susceptible (DS) strain and deltamethrin-resistant (DR) strain using illumina sequencing in Culex pipiens pollens were identified. In this study, we applied RNAi and the Centers for Disease Control and Prevention (CDC) bottle bioassay to investigate the relationship between miR-2 similar to 13 similar to 71 cluster (miR-2, miR-13 and miR-71) and deltamethrin resistance. We used quantitative real-time PCR (qRT-PCR) to measure expression levels of miR-2 similar to 13 similar to 71 clusters. miR-2 similar to 13 similar to 71 cluster was down regulated in adult female mosquitoes from the DR strain and played important roles in deltamethrin resistance through regulating target gene's, CYP9J35 and CYP325BG3. Knocking down CYP9J35 and CYP325BG3 resulted in decreased mortality of DR mosquitoes. This study provides the first evidence that miRNA clusters are associated with deltamethrin resistance in mosquitoes. Moreover, we investigated the regulatory networks formed between miR-2 similar to 13 similar to 71 cluster and its target genes, which provide a better understanding of the mechanism involved in deltamethrin resistance. (C) 2017 Elsevier Ltd. All rights reserved.