Sustained Delivery of BMP-2-Related Peptide from the True Bone Ceramics/Hollow Mesoporous Silica Nanoparticles Scaffold for Bone Tissue Regeneration
Sustained Delivery of BMP-2-Related Peptide from the True Bone Ceramics/Hollow Mesoporous Silica Nanoparticles Scaffold for Bone Tissue Regeneration
复制标题
真骨陶瓷/中空介孔二氧化硅纳米颗粒支架持续输送 BMP-2 相关肽用于骨组织再生
DOI：
10.1021/acsbiomaterials.7b00506
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发表时间：
2018-01-01
影响因子：
5.8
通讯作者：
Guo, Xiaodong
中科院分区：
文献类型：
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作者：
Cui, Wei;Liu, Qianqian;Guo, Xiaodong
骨形态发生蛋白2 （BMP-2）是骨组织形成的重要因子之一。许多BMP-2相关的小分子生物活性肽已经被设计出来，并被证明在成骨活性方面同样有效。在本报告中，我们P28)并设计了一种递送系统来调节P28从真骨陶瓷（TBC）中控释，并结合TBC/HMSN支架中的大孔空心P28比TBC支架中的P28释放慢。采用MC3T3-E1细胞对TBC、TBC/HMSN、TBC/P28支架进行体外细胞实验。结果表明，TBC/HMSN/P28支架对MC3T3-E1细胞增殖和成骨分化的促进作用优于TBC、TBC/HMSN和TBC/P28支架。四种支架分别植入兔桡骨临界骨缺损6周和12周后，x线和组织学检查表明，该成骨递送系统TBC/HMSN/P28支架在体内有效诱导骨再生。因此，TBC/HMSN/P28支架在体外可促进MC3T3-E1细胞增殖和成骨分化，在体内可促进新骨组织生成。该研究为骨组织工程和再生医学提供了一种有前景的支架材料。
Bone morphogenetic protein 2 (BMP-2) is one of the most important factors for bone tissue formation. A number of BMP-2 related small molecule bioactive peptides have been designed and shown to be equally effective in osteogenic activity. In this report, we P28) and designed a delivery system to regulate the controlled release of P28 from true bone ceramics (TBC) combined with an enlarged pore hollow P28 from the TBC/HMSN scaffold was slower than that from the TBC scaffold. An in vitro cell experiment of the TBC/HMSN/P28 scaffold was tested with MC3T3-E1 cells in comparison to TBC, TBC/HMSN, and TBC/P28 scaffolds. Our results demonstrated that the TBC/HMSN/P28 scaffold had better effects on promoting proliferation and osteogenic differentiation of MC3T3-E1 cells than TBC, TBC/HMSN, and TBC/P28 scaffolds. After four kinds of scaffolds were implanted into a rabbit radius critical bone defect for 6 and 12 weeks, the radiographic and histological examination indicated that this osteogenic delivery system TBC/HMSN/P28 scaffold effectively induced bone regeneration in vivo. Therefore, the TBC/HMSN/P28 scaffold can promote proliferation and osteogenic differentiation of MC3T3-E1 cells in vitro and new bone tissue generation in vivo. This study provides a promising scaffold for bone tissue engineering and regenerative medicine.