The role of adhesion molecules in synovial pannus formation in rheumatoid arthritis.
The role of adhesion molecules in synovial pannus formation in rheumatoid arthritis.
复制标题
粘附分子在类风湿性关节炎滑膜血管翳形成中的作用。
DOI：
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发表时间：
1994
影响因子：
4.2
通讯作者：
O. Ohno
中科院分区：
文献类型：
--
作者：
H. Ishikawa;S. Hirata;Y. Nishibayashi;S. Imura;H. Kubo;O. Ohno
为了阐明细胞与软骨结合的机制，使用免疫过氧化物酶技术与抗粘附分子的单克隆抗体，这些分子在类风湿性脂膜软骨连接处的分布模式进行了研究。用纯化的抗人单克隆抗体CD 54抗（ICAM-1）治疗导致大多数浸润滑膜组织和血管翳软骨交界处的大细胞膜染色。用纯化的抗人单克隆抗体CDw 49 d抗（VLA-4）、CDw 49 e抗（VLA-5）处理标本时，软骨血管翳连接处的大部分细胞染色，而抗纯化的抗人单克隆抗体CD 11 a抗（LFA-1）的染色细胞很少。在交界处或其附近的软骨细胞有一些抗ICAM-1和抗VLA-5染色。人脐静脉ECBBA 1（ELAM-1）染色仅见于滑膜组织毛细血管后微静脉内皮细胞。这些结果表明，测试的特异性粘附分子可能在类风湿性血管翳形成中起作用，并且在软骨血管翳连接处VLA-4、VLA-5和ICAM-1的表达增加可能代表与基质蛋白的相互作用。VLA相互作用似乎参与血管翳附着，而LFA-1和ICAM-1参与细胞-细胞相互作用，并可能上调参与附着的分子如VLA。
To elucidate the mechanism of cell binding to cartilage, using an immunoperoxidase technique with monoclonal antibodies against adhesion molecules, the pattern of distribution of these molecules at the rheumatoid pannus-cartilage junction has been investigated. Treatment with purified anti-human-monoclonal antibody CD54 anti-(ICAM-1) resulted in membrane staining of most of the large cells infiltrating the synovial tissue and bordering the pannus cartilage junction. When the specimen was treated with purified anti-human-monoclonal antibody CDw49d anti-(VLA-4), purified anti-human-monoclonal antibody CDw49e anti-(VLA-5), most of the cells in the cartilage pannus junction stained, but there were few staining cells against purified anti-human-monoclonal antibody CD11a anti-(LFA-1). There were some anti-ICAM-1 and anti-VLA-5 staining of the chondrocytes at or close to the junction. Human umbilical vein ECBBA1 (ELAM-1) staining was only observed on the endothelial cells of postcapillary venules in the synovial tissue. These results show that the specific adhesion molecules tested may play a role in rheumatoid pannus formation and that the increased expression of VLA-4, VLA-5, and ICAM-1 at the cartilage pannus junction may represent interaction with matrix protein. The VLA interaction appear to be involved in pannus attachment, whereas LFA-1 and ICAM-1 are involved in cell-cell interaction and may upregulate molecules such as VLA that are involved in attachment.
DOI：
10.1073/pnas.86.24.9906
发表时间：
1989-12-01
影响因子：
11.1
作者：
ELICES, MJ;HEMLER, ME
通讯作者：
HEMLER, ME