Surface plasmon resonance (SPR) sensors for the rapid, sensitive detection of the cellular response to osmotic stress
Surface plasmon resonance (SPR) sensors for the rapid, sensitive detection of the cellular response to osmotic stress
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DOI：
10.1016/j.snb.2011.02.041
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发表时间：
2011-08-01
影响因子：
8.4
通讯作者：
Robelek, R.
中科院分区：
文献类型：
--
作者：
Baumgarten, S.;Robelek, R.
细胞调节它们的体积以响应它们的细胞外和细胞内环境的渗透压的变化。由于细胞体积的稳定性是细胞完整性的迫切需要，用于粘附生长的哺乳动物细胞的灵敏的、时间分辨的体积测量的技术吸引了相当大的兴趣，特别是在细胞生理学和生物学领域。在这项研究中，我们应用表面等离子体共振（SPR）为基础的生物传感器的体积响应的两种肾上皮细胞类型的非等渗挑战的比较分析。在线，无标记和非侵入性的生物传感器格式显示出两种细胞类型的反应动力学的明显的相似性和差异。此外，调节体积的渗透刺激，以及它们的抑制Gd 3+离子的反应，可以观察到具有高的时间分辨率。检测限测量表明该传感器具有高灵敏度，能够检测贴壁生长的哺乳动物细胞对渗透刺激的细胞体积反应，该渗透刺激远低于生物分析相关值5 mOsm/kg。(C)2011 Elsevier B. V.保留所有权利。
Cells regulate their volume in response to changes in osmolarity of both, their extracellular and intracellular environments. As stability of the cell volume is a compelling exigency for cellular integrity, techniques for a sensitive, time-resolved volume measurement of adherently grown mammalian cells attract considerable interest, especially in the field of cell physiology and biology. In this study we apply a surface plasmon resonance (SPR) based biosensor for the comparative analysis of the volume responses of two renal epithelial cell types to non-isotonic challenges. The on-line, label-free and non-invasive biosensor format shows distinct similarities and differences in the reaction kinetics of the two cell types. Furthermore regulatory volume responses to the osmotic stimuli as well as their inhibition by Gd3+ ions can be observed with a high time-resolution. Limit-of-detection measurements indicate the high sensitivity of the sensor capable of detecting cellular volume responses of adherently grown mammalian cells to osmotic stimuli well below a bioanalytical relevant value of 5 mOsm/kg. (C) 2011 Elsevier B.V. All rights reserved.