Cell poking: quantitative analysis of indentation of thick viscoelastic layers.
Cell poking: quantitative analysis of indentation of thick viscoelastic layers.
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DOI：
10.1016/s0006-3495(89)82867-x
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发表时间：
1989-04
影响因子：
3.4
通讯作者：
M. Duszyk;B. Schwab;G. Zahalak;H. Qian;E. Elson
中科院分区：
文献类型：
--
作者：
M. Duszyk;B. Schwab;G. Zahalak;H. Qian;E. Elson
最近引进的一种设备，细胞扑克，测量所需的力量，以使细胞粘附到刚性基质的暴露表面。细胞扑克提供了现象学的信息，几种不同类型的细胞的粘弹性，关于由外部刺激引发的机械变化，以及细胞骨架在这些机械功能中的作用。然而，除了在特殊情况下，它一直不可能提取定量估计的粘度和弹性模量从细胞扑克测量。本文介绍了细胞扑克测量的特点粘弹性聚合物材料，聚二甲基硅氧烷的不同聚合度，在一个简单的形状，一个平面，厚层，这对我们的目的可以被视为半空间。线性粘弹性理论方面的测量结果的分析产生的粘度值为三个聚合物样品在宏观尺度上的测量所确定的那些协议。理论分析进一步表明，所测量的层的极限静态弹性可能是由在聚合物和水之间的界面处产生的张力引起的。这项工作证明了从细胞扑克测量获得定量粘弹性材料特性的可能性，并代表了将这些定量研究扩展到包括细胞在内的更复杂结构的第一步。
A recently introduced device, the cell poker, measures the force required to indent the exposed surface of a cell adherent to a rigid substratum. The cell poker has provided phenomenological information about the viscoelastic properties of several different types of cells, about mechanical changes triggered by external stimuli, and about the role of the cytoskeleton in these mechanical functions. Except in special cases, however, it has not been possible to extract quantitative estimates of viscosity and elasticity moduli from cell poker measurements. This paper presents cell poker measurements of well characterized viscoelastic polymeric materials, polydimethylsiloxanes of different degrees of polymerization, in a simple shape, a flat, thick layer, which for our purposes can be treated as a half space. Analysis of the measurements in terms of a linear viscoelasticity theory yields viscosity values for three polymer samples in agreement with those determined by measurements on a macroscopic scale. Theoretical analysis further indicates that the measured limiting static elasticity of the layers may result from the tension generated at the interface between the polymer and water. This work demonstrates the possibility of obtaining quantitative viscoelastic material properties from cell poker measurements and represents the first step in extending these quantitative studies to more complicated structures including cells.