Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice
Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice
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接种编码针对 CD317 的 HBs 抗原的 DNA 疫苗可增强小鼠的免疫力
DOI：
10.1016/j.bbrc.2018.09.024
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发表时间：
2018-10-12
影响因子：
3.1
通讯作者：
Wan，Xiaochun
中科院分区：
文献类型：
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作者：
Zhang，Guizhong;Li，Xin;Wan，Xiaochun
研究背景常规乙肝疫苗在5-10%的免疫活性疫苗中不能诱导保护性抗体滴度。方法本研究构建了编码乙肝表面抗原CD317单链抗体(α317scFv)的基因疫苗，并检测了该疫苗在小鼠体内诱导的体液免疫和细胞免疫应答。与对照组相比，融合DNA免疫组小鼠血清循环中抗-HBs抗体水平增加了近一倍。更有趣的是，融合基因免疫小鼠的脾淋巴细胞在体外用重组乙肝表面抗原重新刺激后，显示出更强的增殖能力和干扰素-γ的产生。不仅如此，融合DNA疫苗致敏的脾细胞对∼的杀伤活性是对照组的3倍。结论融合DNA疫苗能诱导更有效的免疫保护作用，是预防乙肝病毒感染的一种有前景的候选疫苗。
BackgroundConventional hepatitis B virus (HBV) vaccines fail to induce protective antibody titers in 5–10% of immune-competent vaccines. Therefore, safe and effective HBV vaccines are still clinically needed.MethodsIn this study, we developed a plasmid DNA vaccine encoding CD317 single-chain fragment variable (α317scFv) linked with the hepatitis B surface antigen (HBsAg) and detected the humoral and cellular immune responses elicited by this vaccine in BALB/c mice.ResultsVaccination with this fusion DNA vaccine in BALB/c mice induced more robust antiviral T cell and antibody immunity against HBsAg. Compared with mice vaccinated with control vaccine encoding HBsAg, the level of serum-circulating anti-HBsAg antibody (HBsAb) was nearly double in fusion DNA-vaccinated mice. More interesting, splenic lymphocytes isolated from fusion DNA-vaccinated mice showed more potent proliferation and IFN-γ production after being re-stimulated with recombinant HBsAgin vitro. And not only that, the cytotoxicity of fusion DNA vaccine-sensitized splenocytes was ∼3-fold higher than that of controls.ConclusionTaken together, our results reveal that the fusion DNA vaccine can induce more effective immunological protection against HBV, and is a promising candidate for preventing HBV infection.