Involvement of Na+-Ca2+ exchanger on metabotropic glutamate receptor 1-mediated [Ca2+]i transients in rat cerebellar purkinje neurons
Involvement of Na+-Ca2+ exchanger on metabotropic glutamate receptor 1-mediated [Ca2+]i transients in rat cerebellar purkinje neurons
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DOI：
10.1016/j.neuroscience.2007.01.040
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发表时间：
2007-04-25
期刊：
影响因子：
3.3
通讯作者：
Suh, C. K.
中科院分区：
文献类型：
--
作者：
Kim, Y. T.;Namkling, Y. L.;Suh, C. K.
小脑浦肯野神经元具有细胞内调节系统，包括 Ca2+ 结合蛋白、细胞内 Ca2+ 储存、Ca2+-ATP 酶和 Na+-Ca2+ 交换器 (NCX)，使细胞内 Ca2+ 浓度 ([Ca2+](i)) 保持在生理范围内。其中，NCX 与 AMPA 受体相互作用，激活 AMPA 受体可诱导小脑突触可塑性。代谢型谷氨酸受体1（mGluR1）的激活也参与小脑长期抑郁的诱发。 NCX 与 mGluR1 的相互作用尚不清楚。因此，在本研究中，研究了 NCX 和 mGluR1 在调节大鼠浦肯野神经元中 [Ca2+](i) 的功能关系。通过测量 I 组 mGluR 激动剂 3,5-二羟基苯基甘氨酸 (DHPG) 诱导的细胞内 Ca2+ 瞬变，研究了浦肯野神经元中 NCX 和 mGluR1 之间的相互作用。通过 NCX 抑制剂、bepridil 和 KB-R7943 的治疗，DHPG 诱导的 Ca2+ 瞬变显着减少。当细胞用NCX反义寡脱氧核苷酸预处理时，DHPG诱导的Ca2+瞬变也被抑制。这些结果表明 NCX 调节小脑浦肯野神经元中 mGIuR1 的活性。因此，NCX似乎在小脑浦肯野神经元的突触可塑性等生理功能中发挥着重要作用。 (c) 2007 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Cerebellar Purkinje neurons have intracellular regulatory systems including Ca2+-binding proteins, intracellular Ca2+ stores, Ca2+-ATPase and Na+-Ca2+ exchanger (NCX) that keep intracellular Ca2+ concentration ([Ca2+](i)) in physiological range. Among these, NCX interacts with AMPA receptors, activation of which induces cerebellar synaptic plasticity. And the activation of metabotropic glutamate receptor 1 (mGluR1) is also involved in the induction of cerebellar long-term depression. The interaction of NCX with mGluR1 is not known yet. Thus, in this study, the functional relationship between NCX and mGluR1 in modulating the [Ca2+](i) in rat Purkinje neurons was investigated. The interaction between NCX and mGluR1 in Purkinje neurons was studied by measuring intracellular Ca2+ transients induced by an agonist of group I mGluRs, 3,5-dihydroxyphenylglycine (DHPG). The DHPG-induced Ca2+ transient was significantly reduced by treatments of NCX inhibitors, bepridil and KB-R7943. When cells were pretreated with antisense oligodeoxynucleotides of NCX, the DHPG-induced Ca2+ transient was also inhibited. These results suggest that NCX modulates the activity of mGIuR1 in cerebellar Purkinje neurons. Therefore, NCX appears to play an important role in the physiological function of cerebellar Purkinje neurons such as synaptic plasticity. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.