Diversity and molecular evolution of the RPS2 resistance gene in Arabidopsis thaliana
Diversity and molecular evolution of the RPS2 resistance gene in Arabidopsis thaliana
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DOI：
10.1073/pnas.96.1.302
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发表时间：
1999-01-05
影响因子：
11.1
通讯作者：
Kunkel, BN
中科院分区：
文献类型：
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作者：
Caicedo, AL;Schaal, BA;Kunkel, BN
拟南芥中的RPS2基因控制着对表达avrRpt2基因的番茄细菌性病原菌的抗性。这两个基因座参与了基因对基因的相互作用。对17份拟南芥RPS2基因座的编码区进行了测序。发现了异常高水平的核苷酸多态(1.26%)，近一半的观察到的多态导致RPS2蛋白的氨基酸变化。共鉴定出7个单倍型(等位基因)，并推测了它们之间的进化关系。几个与抗性有关的等位基因被发现是密切相关的，而对疾病的敏感性则是由差异很大的等位基因决定的。讨论了在RPS2基因座进行选择的可能性。
The RPS2 gene in Arabidopsis thaliana governs resistance to strains of the bacterial pathogen, Pseudomonas syringae pv, tomato, that express the avrRpt2 gene. The two loci are involved in a gene-for-gene interaction. Seventeen accessions of A. thaliana were sequenced to explore the diversity present in the coding region of the RPS2 locus. An unusually high level of nucleotide polymorphisms was Found (1.26%), with nearly half of the observed polymorphisms resulting in amino acid changes in the RPS2 protein. Seven haplotypes (alleles) were identified and their evolutionary relationships deduced. Several of the alleles conferring resistance were found to be closely related, whereas susceptibility to disease was conferred by widely divergent alleles. The possibility of selection at the RPS2 locus is discussed.