Synthetic hepatitis B surface antigen peptide vaccine.
Synthetic hepatitis B surface antigen peptide vaccine.
复制标题
合成乙型肝炎表面抗原肽疫苗。
DOI：
10.1007/978-1-4684-7974-4_8
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发表时间：
1985
影响因子：
--
通讯作者：
Kennedy,RC
中科院分区：
文献类型：
--
作者：
Dreesman,GR;Sparrow,JT;Frenchick,PJ;Kennedy,RC
A synthetic hepatitis B surface antigen (HBsAg) peptide was prepared containing amino acid residues 122–137 of the major HBsAg polypeptide. This peptide was cyclized by the introduction of an intrachain disulfide bond between cysteine residues at positions 124 and 137 because previous studies had shown that intact disulfide bonds are critical for maintenance of HBsAg activity. An anti-HBs response was produced in mice by free peptide entrapped in liposomes. However, the immunogenicity was enhanced by aggregation into micelles, and by coupling to tetanus toxoid. Analysis of the peptide with a panel of monoclonal antibodies showed that peptide 122–137 contained a conformation (discontinuous) groupaepitope and a sequential (continuous) subgroupyepitope. In addition, the cyclic peptide inhibited a human anti-HBs idiotype-antiidiotype reaction with specificity for groupadeterminant(s). The potential for synthetic peptides for hepatitis B virus vaccine development is discussed.
