Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Caenorhabditis elegans Lysates
Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Caenorhabditis elegans Lysates
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DOI：
10.21769/bioprotoc.3042
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发表时间：
2018-10-05
期刊：
影响因子：
0.8
通讯作者：
Nollen, Ellen A. A.
中科院分区：
文献类型：
--
作者：
Sin, Olga;Mata-Cabana, Alejandro;Nollen, Ellen A. A.
蛋白质聚集是几种神经退行性疾病的标志，并且与受损的蛋白质稳态相关。这种不平衡是由蛋白质的天然构象的丧失引起的，这最终导致其在细胞内的聚集或异常定位。使用C。在多聚谷氨酰胺疾病的elegans模型中，我们详细描述了过滤阻滞测定，这是一种在醋酸纤维素膜中捕获蛋白质聚集体并通过免疫印迹对其进行检测和定量的方法。
Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein's native conformation, which ultimately results in its aggregation or abnormal localization within the cell. Using a C. elegans model of polyglutamine diseases, we describe in detail the filter retardation assay, a method that captures protein aggregates in a cellulose acetate membrane and allows its detection and quantification by immunoblotting.