Identification and quantitation of ascorbic acid in extracts of human lymphocytes by high-performance liquid chromatography.
Identification and quantitation of ascorbic acid in extracts of human lymphocytes by high-performance liquid chromatography.
复制标题
通过高效液相色谱法鉴定和定量人淋巴细胞提取物中的抗坏血酸。
DOI：
10.1016/0003-2697(81)90155-x
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发表时间：
1981
影响因子：
2.9
通讯作者：
Silber,R
中科院分区：
文献类型：
--
作者：
Liebes,LF;Kuo,S;Krigel,R;Pelle,E;Silber,R
60% 的甲醇广泛用于从淋巴细胞中提取核苷酸，以便通过高效液相色谱进行定量。在此类研究过程中，我们注意到在阴离子交换柱上分析的这些提取物显示出主要的“未知”紫外线吸收峰，该峰在核苷之后和核苷酸之前洗脱。该材料与抗坏血酸进行共色谱分析并具有抗坏血酸的光谱特性。通过化学和酶测定，该化合物被鉴定为抗坏血酸。通过高效液相色谱法测定的人淋巴细胞的抗坏血酸含量为 42.2 ± 3.3 nmol 108 个细胞（平均值 ± SEM），与通过标准不太敏感的方法获得的水平非常一致。有证据表明，该技术可用于测定淋巴细胞的抗坏血酸含量，而淋巴细胞中的抗坏血酸含量很少或水平很低。
Sixty percent methanol is widely used for the extraction of nucleotides from lymphocytes for quantitation by high-performance liquid chromatography. In the course of such studies, we noted that these extracts analyzed on an anion-exchange column showed a major “unknown” uv-absorbing peak which eluted after the nucleosides and before the nucleotides. The material cochromatographed with and had the spectral properties of ascorbic acid. This compound was identified as ascorbic acid by chemical and enzymatic assays. The ascorbate content of human lymphocytes determined by high-performance liquid chromatography, 42.2 ± 3.3 nmol 108cells (mean ± SEM), agreed closely with the levels obtained by standard less sensitive methodology. Evidence is presented that this technique can be used to determine the ascorbate content of lymphocytes where only scanty material or very low levels are found.