Further evidence for the presence of specific binding sites for prolactin in the rabbit brain. Preferential distribution in the hypothalamus and substantia nigra.
Further evidence for the presence of specific binding sites for prolactin in the rabbit brain. Preferential distribution in the hypothalamus and substantia nigra.
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兔脑中存在催乳素特异性结合位点的进一步证据。
DOI：
10.1016/0024-3205(85)90124-9
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发表时间：
1985
期刊：
影响因子：
6.1
通讯作者：
G. Bellussi
中科院分区：
文献类型：
--
作者：
R. Di Carlo;G. Muccioli;D. Lando;G. Bellussi
在目前的研究中，我们扩展了对兔脑中催乳素结合位点存在的研究，将我们的注意力集中在具有高多巴胺细胞体密度的大脑区域。在这些区域中，下丘脑显示出标记的绵羊催乳素（oPRL）的最高特异性结合。在黑质中也观察到明显可检测到的特异性结合，而在其他脑区域中，除了纹状体之外，特异性结合非常小，在纹状体中在雌性兔子中发现了低但不可忽略的结合。125I-oPRL的结合显示出激素特异性，并且结合的斯卡查德分析显示下丘脑、黑质和纹状体之间的解离常数(Kd)没有明显差异。
In the present research we have extended our work on the presence of binding sites for prolactin in the rabbit brain focusing our attention on the brain areas with high dopamine cell bodies density. Among these areas the hypothalamus showed the highest specific binding of labeled ovine prolactin (oPRL). Clearly detectible specific binding was observed also in substantia nigra, whereas in other brain regions the specific binding was very small, except for the striatum where a low but not negligible binding was found in female rabbits.The binding of125I-oPRL showed a hormonal specificity and Scatchard analysis of the binding showed no clear difference in dissociation constant (Kd) between hypothalamus, nigra and striatum.