Optimal biomaterial for creation of autologous cardiac grafts
Optimal biomaterial for creation of autologous cardiac grafts
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DOI：
10.1161/01.cir.0000032901.55215.cc
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发表时间：
2002-09-24
期刊：
影响因子：
37.8
通讯作者：
Li, RK
中科院分区：
文献类型：
--
作者：
Ozawa, T;Mickle, DAG;Li, RK
背景-用于修复先天性心脏缺陷的最佳心脏移植物将由自体细胞组成，并与孩子一起生长。可生物降解材料应允许细胞快速生长和延迟降解，同时炎症最小化。我们将一种新材料，用针织聚 L-丙交酯织物 (PCLA) 增强的 ε-己内酯-共-L-丙交酯海绵，与之前评估过的材料明胶 (GEL) 和聚乙醇酸 (PEA) 进行比较。 方法-将同源大鼠主动脉平滑肌细胞 (SMCs, 2X10(6)) 接种到 GEL、PEA 和 PCLA 贴片上并培养 （每组 n=11）。在播种后 1、2 和 3 周测量每个斑块中的 DNA 含量。接种后2周进行组织学检查。采用细胞接种贴片来替代手术造成的大鼠右心室流出道 (RVOT) 缺损（每组 n=5）。植入后8周进行组织学研究。结果-体外研究表明DNA含量显着增加（P
Background-The optimal cardiac graft for the repair of congenital heart defects will be composed of autologous cells and will grow with the child. The biodegradable material should permit rapid cellular growth and delayed degradation with minimal inflammation. We compared a new material, epsilon-caprolactone-co-L-lactide sponge reinforced with knitted poly-L-lactide fabric (PCLA), to gelatin (GEL) and polyglycolic acid (PEA), which are previously evaluated materials.Methods-Syngenic rat aortic smooth muscle cells (SMCs, 2X10(6)) were seeded onto GEL, PEA, and PCLA patches and cultured (n=11 per group). The DNA content in each patch was measured at 1, 2, and 3 weeks after seeding. Histological examination was performed 2 weeks after seeding. Cell-seeded patches were employed to replace a surgically created defect in the right ventricular outflow tract (RVOT) of rats (n=5 per group). Histology was studied at 8 weeks following implantation.Results-In vitro studies showed that the DNA content increased significantly (P