Lateral resolution enhancement of laser scanning microscopy by a higher-order radially polarized mode beam
Lateral resolution enhancement of laser scanning microscopy by a higher-order radially polarized mode beam
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DOI：
10.1364/oe.19.015947
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发表时间：
2011-08-15
期刊：
影响因子：
3.8
通讯作者：
Sato, Shunichi
中科院分区：
文献类型：
--
作者：
Kozawa, Yuichi;Hibi, Terumasa;Sato, Shunichi
我们表明，共焦激光扫描显微镜的横向分辨率显着提高了高阶径向偏振（HRP）光束与六个同心环。该光束简单地通过在物镜透镜前面插入液晶器件而产生。HRP束可视化聚集的0.17 μ m的珠粒，也适用于生物成像。这种方法可以扩展传统激光扫描显微镜的能力，而无需修改系统，除了在光路中添加液晶器件。（C）2011年美国光学学会
We demonstrate that the lateral resolution of confocal laser scanning microscopy is dramatically improved by a higher-order radially polarized (HRP) beam with six concentric rings. This beam was generated simply by inserting liquid crystal devices in front of an objective lens. An HRP beam visualized aggregated 0.17 mu m beads individually and is also applicable to biological imaging. This method can extend the capability of conventional laser scanning microscopes without modification of the system, with the exception of the addition of the liquid crystal devices in the optical path. (C)2011 Optical Society of America