Multigene phylogeny resolves deep branching of Amoebozoa
Multigene phylogeny resolves deep branching of Amoebozoa
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DOI：
10.1016/j.ympev.2014.08.011
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发表时间：
2015-02-01
影响因子：
4.1
通讯作者：
Lewis, Rhodri
中科院分区：
文献类型：
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作者：
Cavalier-Smith, Thomas;Fiore-Donno, Anna Maria;Lewis, Rhodri
阿米巴虫是真核生物发生和进化史上的一个关键门，但其系统发育的有效性受到质疑，因为包括种类非常多样：阿米巴鞭毛虫黏霉菌，裸和有遗嘱的阿米巴，以及一些鞭毛虫。18 S rRNA基因树还没有牢固地建立其内部拓扑结构。为了纠正这一点，我们测序了7个不同的阿米巴虫的cDNA文库，并用60-188个基因对109个真核生物（17-18个阿米巴虫）进行了系统发育分析。我们使用进化上最现实的站点异质CAT-GTR-Gamma模型和最大似然分析进行了贝叶斯推断。这些明确地建立了变形虫的单系性，显示了以前有争议的叶瓣亚门和芋螺亚门之间的主要二分法。Lobosa，完全无鞭毛的叶状变形虫，被强烈地划分为单系纲Tubulocytes，主要是管状伪足，和Discosea，扁平细胞和不同的运动。在芋螺60/70基因树很少缺失的数据之间的主要二分法有氧infraphylum Semicosia（Mycetozoa和Variosea）和其次厌氧Archamoeulae。这些系统发育特征是完全一致的，最近的主要阿米巴分类强调运动模式，伪足形态，超微结构。然而，188个基因的树，按比例更多的分类群有稀疏的基因表示弱的地方Archamoeastrum的姐妹，而不是大型真菌，可能是一个树重建文物的差异缺失的数据。(C)2014作者爱思唯尔公司出版这是CC BY许可下的开放获取文章（http：//creativecommons.org/licenses/by/3.0/）。
Amoebozoa is a key phylum for eukaryote phylogeny and evolutionary history, but its phylogenetic validity has been questioned since included species are very diverse: amoebo-flagellate slime-moulds, naked and testate amoebae, and some flagellates. 18S rRNA gene trees have not firmly established its internal topology. To rectify this we sequenced cDNA libraries for seven diverse Amoebozoa and conducted phylogenetic analyses for 109 eukaryotes (17-18 Amoebozoa) using 60-188 genes. We conducted Bayesian inferences with the evolutionarily most realistic site-heterogeneous CAT-GTR-Gamma model and maximum likelihood analyses. These unequivocally establish the monophyly of Amoebozoa, showing a primary dichotomy between the previously contested subphyla Lobosa and Conosa. Lobosa, the entirely non-flagellate lobose amoebae, are robustly partitioned into the monophyletic classes Tubulinea, with predominantly tube-shaped pseudopodia, and Discosea with flattened cells and different locomotion. Within Conosa 60/70-gene trees with very little missing data show a primary dichotomy between the aerobic infraphylum Semiconosia (Mycetozoa and Variosea) and secondarily anaerobic Archamoebae. These phylogenetic features are entirely congruent with the most recent major amoebozoan classification emphasising locomotion modes, pseudopodial morphology, and ultrastructure. However, 188-gene trees where proportionally more taxa have sparser gene-representation weakly place Archamoebae as sister to Macromycetozoa instead, possibly a tree reconstruction artefact of differentially missing data. (C) 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).