DIFFERENTIAL ROLES OF RYANODINE‐ AND THAPSIGARGIN‐SENSITIVE INTRACELLULAR Ca2+ STORES IN EXCITATION–CONTRACTION COUPLING IN SMOOTH MUSCLE OF GUINEA‐PIG TAENIA CAECI
DIFFERENTIAL ROLES OF RYANODINE‐ AND THAPSIGARGIN‐SENSITIVE INTRACELLULAR Ca2+ STORES IN EXCITATION–CONTRACTION COUPLING IN SMOOTH MUSCLE OF GUINEA‐PIG TAENIA CAECI
复制标题
兰尼碱和毒胡萝卜素敏感的细胞内 Ca2+ 储存在豚鼠平滑肌兴奋-收缩耦合中的不同作用
DOI：
10.1111/j.1440-1681.2006.04506.x
复制
发表时间：
2006
影响因子：
2.9
通讯作者：
Masaki Saito
中科院分区：
文献类型：
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作者：
S. Hishinuma;Masaki Saito
1为了探讨细胞内钙库在平滑肌兴奋-收缩偶联中的作用，我们研究了在存在和不存在细胞外钙的情况下，Ryanodine（Ryanodine受体-Ca 2+通道开放状态的固定剂）和thapsigargin（细胞内钙库中Ca 2+泵的选择性抑制剂）对豚鼠盲肠带平滑肌收缩的影响。2在无钙溶液中，1 μmol/L毒胡萝卜素或10 μmol/L ryanodine可使0.1 mmol/L卡巴胆碱和0.1 mmol/L组胺诱导的收缩减少至对照的约65%。相比之下，咖啡因诱导的收缩被ryanodine减少到对照的约40%，但不受毒胡萝卜素的影响。3在细胞外Ca ~（2+）存在下，毒胡萝卜素缓慢地引起大而持续的收缩。相反，ryanodine没有引起明显的收缩，但增加了收缩反应对受体激动剂（卡巴胆碱，AHR-602和组胺）或去极化高K+的敏感性，而最大收缩没有变化。4这些结果表明，在平滑肌兴奋-收缩偶联中，ryanodine和thapsigargin敏感的细胞内Ca 2+库之间存在药理学和生理学差异，这可能是它们对Ca 2+内流途径的不同影响的原因。
1 To explore roles of intracellular Ca2+ stores in excitation–contraction coupling in smooth muscle, we examined the effects of ryanodine, a fixer of ryanodine receptor–Ca2+ channels to an open state, and thapsigargin, a selective inhibitor of the Ca2+ pump in the intracellular stores, on smooth muscle contraction in the presence and absence of extracellular Ca2+ in guinea‐pig taenia caeci. 2 In Ca2+‐free solution, contractions induced by 0.1 mmol/L carbachol and 0.1 mmol/L histamine were reduced to approximately 65% of control by either 1 µmol/L thapsigargin or 10 µmol/L ryanodine. In contrast, caffeine‐induced contraction was reduced to approximately 40% of control by ryanodine, but was not affected by thapsigargin. 3 In the presence of extracellular Ca2+, thapsigargin slowly induced a large and sustained contraction. In contrast, ryanodine did not induce an apparent contraction, but increased the sensitivity of contractile responses to receptor agonists (carbachol, AHR‐602 and histamine) or depolarizing high K+ with no changes in the maximal contraction. 4 These results suggest that there are pharmacological and physiological differences between ryanodine‐ and thapsigargin‐sensitive intracellular Ca2+ stores in excitation–contraction coupling in smooth muscle, which may be responsible for their differential effects on the Ca2+‐influx pathway.
DOI：
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发表时间：
2006
期刊：
American journal of physiology. Cell physiology
影响因子：
--
作者：
J. Mauban;K. Wilkinson;C. Schach;J. Yuan
通讯作者：
J. Mauban;K. Wilkinson;C. Schach;J. Yuan
Ryanodine 对兔主动脉平滑肌 45Ca 流出和张力的调节。
DOI：
10.1007/bf00581127
发表时间：
1987
期刊：
Pflugers Archiv : European journal of physiology
影响因子：
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作者：
Hwang,KS;vanBreemen,C
通讯作者：
vanBreemen,C