CHROMOSOME SITE FOR EPSTEIN-BARR VIRUS-DNA IN A BURKITT TUMOR-CELL LINE AND IN LYMPHOCYTES GROWTH-TRANSFORMED INVITRO
CHROMOSOME SITE FOR EPSTEIN-BARR VIRUS-DNA IN A BURKITT TUMOR-CELL LINE AND IN LYMPHOCYTES GROWTH-TRANSFORMED INVITRO
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DOI：
10.1073/pnas.80.7.1987
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
KIEFF, E
中科院分区：
文献类型：
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作者：
HENDERSON, A;RIPLEY, S;KIEFF, E
Epstein-Barr 病毒 (EBV) 基因组稳定地存在于潜伏感染的人伯基特肿瘤细胞和生长转化的 B 淋巴细胞中。这些细胞通常含有多个附加型病毒 DNA 拷贝。重组病毒 DNA 片段与 2 个潜伏感染细胞系的中期染色体的细胞学杂交表明，病毒 DNA 定位于 Burkitt 肿瘤细胞系 Namalwa 中 1 号染色体的 1 个同源物的两个染色单体，以及 IB4（一种体外具有转化生长特性的细胞系）中 4 号染色体的 1 个同源物的两个染色单体。 IB4 细胞克隆中染色体关联位点保持稳定。来自 EBV 基因组 5 个不同区域的探针与相同的染色体区域杂交。在 EBV 染色体细胞学杂交区域内发现了一个先前未描述的无色位点。这些观察结果表明，大部分或全部 EBV 基因组整合到 Namalwa 和 IB4 细胞的染色体 DNA 中。尽管 EBV DNA 关联的染色体位点位于与 EBV IR3 重复 DNA 序列同源的区域中，但 EBV IR3 并不介导 EBV 和染色体 DNA 之间的重组。
The Epstein-Barr virus (EBV) genome stably persists in latently infected human Burkitt tumor cells and growth-transformed B lymphocytes. These cells usually contain multiple copies of episomal viral DNA. Cytological hybridization of recombinant viral DNA fragments to metaphase chromosomes of 2 latently infected cell lines demonstrates that viral DNA localizes to both chromatids of 1 homolog of chromosome 1 in Namalwa, a Burkitt tumor cell line, and to both chromatids of 1 homolog of chromosome 4 in IB4, a cell line with transformed growth properties in vitro. The site of chromosome association remains stable in a clone of IB4 cells. Probes from 5 separate regions of the EBV genome hybridize to the same chromosome regions. A previously undescribed achromatic site is identified within the region of EBV chromosome cytological hybridization. These observations suggest that most or all of the EBV genome is integrated into the chromosomal DNA of Namalwa and IB4 cells. Although the chromosomal sites of EBV DNA association are among those regions with homology to the EBV IR3 repeated DNA sequence, EBV IR3 did not mediate recombination between EBV and chromosomal DNA.