Two Sec13p Homologs, AtSec13A and AtSec13B, Redundantly Contribute to the Formation of COPII Transport Vesicles in Arabidopsis thaliana

Two Sec13p Homologs, AtSec13A and AtSec13B, Redundantly Contribute to the Formation of COPII Transport Vesicles in Arabidopsis thaliana
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DOI:
10.1271/bbb.110331
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发表时间:
2011-09-01
影响因子:
1.6
通讯作者:
Nakagawa, Tsuyoshi
Nakagawa, Tsuyoshi
中科院分区:
工程技术4区
文献类型:
--
作者:
Hino, Takeshi;Tanaka, Yuji;Nakagawa, Tsuyoshi

文献摘要

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COPII囊泡介导蛋白质从ER转运到高尔基体。Sec13与Sec31组成晶格结构,形成COPII囊泡。我们分析了两个拟南芥Sec13同源物AtSec13A和AtSec13B的表达。AtSec13A在幼苗的大部分部位表达,AtSec13B部分表达。通过双分子荧光互补(BiFC)证实了AtSec13A或AtSec13B与Sec31同源物的相互作用。
COPII vesicles mediate protein transport from ER to Golgi. Sec13 makes up lattice structure with Sec31 to form COPII vesicles. We analyzed expression of two Arabidopsis thaliana Sec13 homologs, AtSec13A and AtSec13B. AtSec13A was expressed in most parts of seedlings, while AtSec13B was partially expressed. Interaction of AtSec13A or AtSec13B with Sec31 homolog was demonstrated by bimolecular fluorescence complementation (BiFC).