SPECIFIC BINDING OF HIV-1 RECOMBINANT REV PROTEIN TO THE REV-RESPONSIVE ELEMENT INVITRO
SPECIFIC BINDING OF HIV-1 RECOMBINANT REV PROTEIN TO THE REV-RESPONSIVE ELEMENT INVITRO
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DOI：
10.1038/342816a0
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发表时间：
1989-12-14
期刊：
影响因子：
64.8
通讯作者：
RUSCHE, JR
中科院分区：
文献类型：
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作者：
DALY, TJ;COOK, KS;RUSCHE, JR
人类免疫缺陷病毒1型（HIV-1）基因组编码相对分子质量为13，000的调节蛋白Rev，该蛋白在HIV-1结构蛋白合成前由完全加工的病毒转录物合成1 -3。据推测，Rev在信使RNA加工水平上在细胞核内发挥控制作用4 -7。相对于完全剪接的mRNA分子，细胞核中Rev的可用性有助于增加未剪接和单剪接的mRNA种类的比例，从而导致病毒结构蛋白的合成增加。在病毒转录物的包膜基因（env）中发现了一个高度保守的顺式作用序列，称为Rev反应元件（RRE），它似乎以Rev依赖性方式控制mRNA加工8 -11。遗传学研究已经鉴定出具有显性表型的Rev基因突变体，支持Rev直接与RRE 12相互作用的假设。在这里，我们证明Rev蛋白，纯化大肠杆菌，结合在一个序列特异性的方式RRE元件在体外。
THE human immunodeficiency virus type 1 (HIV-1) genome encodes the regulatory protein Rev, of relative molecular mass 13,000, which is synthesized from fully processed viral transcripts before synthesis of HIV-1 structural proteins1–3. Rev has been postulated to exert control within the nucleus at the level of messenger RNA processing4–7. The availability of Rev in the nucleus serves to increase the proportion of unspliced and singly spliced mRNA species relative to fully spliced mRNA molecules, resulting in an increased synthesis of viral structural proteins. A highly conservedcis-acting sequence termed the Rev-responsive element (RRE) has been identified in the envelope gene (env) of the viral transcript that seems to control mRNA processing in a Rev-dependent manner8–11. Genetic studies have identifiedrevgene mutants with dominant phenotypes, supporting the hypothesis that Rev interacts directly with the RRE12. Here we demonstrate that Rev protein, purified fromEscherichia coli, binds in a sequence-specific manner to the RRE elementin vitro.