Calcification of rachitic cartilage to study matrix vesicle function.
Calcification of rachitic cartilage to study matrix vesicle function.
复制标题
佝偻软骨钙化研究基质囊泡功能。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
Sajdera Sw
中科院分区：
文献类型：
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作者：
Anderson Hc;Sajdera Sw
研究佝偻病大鼠的生长板软骨，以评估细胞外基质囊泡在愈合过程中钙化重建中的作用。基质囊泡的浓度和分布被认为是正常的佝偻病生长板，虽然佝偻病软骨基质在很大程度上未钙化，偶尔囊泡不包含内部mineral. Matrix囊泡作为初始位点矿化愈合时，无论是在体内注射磷酸盐或在体外培养的生长板在亚稳态钙化溶液。在体外钙化过程中，在上生长板中形成了一条明显的矿化线，电子显微镜显示这反映了囊泡的矿化。这种囊泡相关钙化线的出现被抑制预热或反复冻融，并通过30分钟的预孵育在脱氧胆酸盐，乙烷-1-羟基-1，1-二膦酸盐，或硫酸铍。我们的研究结果表明，囊泡钙化是依赖于囊泡膜的结构和酶的完整性。可能在囊泡钙化中起作用的酶是磷酸酶（例如，例如，在一个实施例中，碱性磷酸酶、焦磷酸酶和ATP酶），已知其浓缩在囊泡膜中。
: Growth plate cartilage from rachitic rats was studied to assess the role of extra-cellular matrix vesicles in the reinstitution of calcification during healing. The concentration and distribution of matrix vesicles was found to be normal in rachitic growth plate, and although the rachitic cartilage matrix was largely uncalcified, an occasional vesicle did contain internal mineral. Matrix vesicles served as initial loci for mineralization when healing was brought about either by in vivo injection of phosphate or in vitro incubation of growth plates in a metastable calcifying solution. During in vitro calcification a distinct line of mineralization developed in the upper growth plate which was shown by electron microscopy to reflect mineralization by the vesicles. The appearance of this vesicle-associated calcification line was inhibited by preheating or repeated freezing and thawing, and by 30 minutes preincubation in deoxycholate, ethane-1-hydroxy-1,1-diphosphonate, or beryllium sulfate. Our results suggest that vesicle calcification is dependent on the structural and enzymatic integrity of the vesicle membrane. Enzymes that may well play a role in vesicle calcification are phosphatases (e. g., alkaline phosphatase, pyrophosphatase and ATPase), which are known to be concentrated in vesicle membranes.