MUTANTS OF ESCHERICHIA-COLI DEFECTIVE IN MEMBRANE PHOSPHOLIPID SYNTHESIS - MAPPING OF STRUCTURAL GENE FOR L-GLYCEROL 3-PHOSPHATE DEHYDROGENASE
MUTANTS OF ESCHERICHIA-COLI DEFECTIVE IN MEMBRANE PHOSPHOLIPID SYNTHESIS - MAPPING OF STRUCTURAL GENE FOR L-GLYCEROL 3-PHOSPHATE DEHYDROGENASE
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DOI：
10.1128/jb.118.2.598-605.1974
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发表时间：
1974-01-01
影响因子：
3.2
通讯作者：
BELL, RM
中科院分区：
文献类型：
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作者：
CRONAN, JE;BELL, RM
生物合成甘油3-磷酸脱氢酶的结构基因已定位于大肠杆菌染色体上的min71.5。该基因（gpsA）与thexyl、mtl和pyrE位点共转导。三因子接合杂交和转导数据表明，染色体该区域的基因座顺序为mtl、gltE、gpsA、gadR、gadS、pyrE。对具有增加的热不稳定性的脱氢酶的温度敏感的gpsA突变体的研究表明gpsA是脱氢酶的结构基因。所有供试的β-淀粉酶缺陷菌株都被定位在非常接近gpsA基因座的位置。基因互补分析的尝试是不成功的。
The structural gene for the biosyntheticl-glycerol 3-phosphate dehydrogenase has been mapped at min 71.5 on theEscherichia colichromosome. This gene (gpsA) is co-transduced with thexyl, mtl, andpyrEloci. Three-factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosone ismtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature-sensitivegpsAmutant possessing a dehydrogenase of increased thermolability indicated thatgpsAis the structural gene for the dehydrogenase. All dehydrogenase-deficient strains tested were mapped very close to thegpsAlocus. Attempts at genetic complementation analysis were unsuccessful.