Increased activity and expression of gelatinases in ischemic colitis
Increased activity and expression of gelatinases in ischemic colitis
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DOI：
10.1007/s10620-006-9255-5
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发表时间：
2006-12-01
影响因子：
3.1
通讯作者：
Quintero, Enrique
中科院分区：
文献类型：
--
作者：
Medina, Carlos;Santana, Alfredo;Quintero, Enrique
缺血性结肠炎是由血液供应不足引起的，但其发病机制尚不清楚。本研究的目的是确定明胶酶（MMP-9和MMP-2）的活性和表达是否在缺血性结肠炎患者的结肠粘膜中增加。MMP-9和MMP-2的活性和表达进行了评估，结肠粘膜标本8例急性缺血性结肠炎和12例正常结肠镜检查的对照组。分别通过酶谱法和蛋白质印迹法定量组织样品中MMP-9和MMP-2的活性和表达。两名患者在出院后12周重复结肠镜检查，并在缺血性结肠炎的轻微残留粘膜变化中评估MMP活性。在缺血性结肠炎患者中，总MMP-9和MMP-2的活性和表达显着增加，发现在溃疡面积与非参与网站的粘膜相比。缺血性溃疡消退后，蛋白水解活性恢复至基线水平。此外，对照组结肠粘膜显示MMP-2活性，而MMP-9活性可忽略或未检测到。我们的结论是，缺血性结肠炎诱导增加的活性和MMP-9和MMP-2的表达在涉及的结肠粘膜。这些变化可能有助于缺血性结肠炎中结肠粘膜的组织降解和重塑。
Ischemic colitis results from insufficient blood supply but its pathogenesis is poorly understood. The aim of this study was to determine whether the activity and expression of gelatinases (MMP-9 and MMP-2) are increased in the colonic mucosa of patients with ischemic colitis. MMP-9 and MMP-2 activity and expression were assessed in colonic mucosal specimens from 8 patients with acute ischemic colitis and in 12 controls with a normal colonoscopy. The activity and expression of MMP-9 and MMP-2 were quantified in tissue samples by zymography and western blot, respectively. Colonoscopy was repeated 12 weeks after discharge in two patients and MMP activity was assessed in the slight residual mucosal changes of ischemic colitis. In patients with ischemic colitis, a significant increase in total MMP-9 and MMP-2 activity and expression was found in ulcerated areas compared with noninvolved sites of mucosa. Following resolution of ischemic ulcers the proteolytic activity returned to baseline levels. In addition, the colonic mucosa of controls showed MMP-2 activity, whereas the MMP-9 activity was negligible or not detected. We conclude that ischemic colitis induces increased activity and expression of MMP-9 and MMP-2 in the involved colonic mucosa. These changes may contribute to tissue degradation and remodeling of the colonic mucosa in ischemic colitis.