c-Fos regulates hepatitis C virus propagation
c-Fos regulates hepatitis C virus propagation
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DOI：
10.1016/j.febslet.2011.08.041
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发表时间：
2011-10-20
期刊：
影响因子：
3.5
通讯作者：
Hwang, Soon B.
中科院分区：
文献类型：
--
作者：
Kang, Sang-Min;Lim, Seri;Hwang, Soon B.
丙型肝炎病毒（HCV）RNA复制需要细胞因子以及病毒非结构蛋白（NS蛋白）。使用小干扰RNA（siRNA）库筛选，我们以前确定的c-Fos作为一个主机的因素参与HCV的传播。在本研究中，我们证明了c-Fos表达的沉默导致在细胞培养生长的HCV（HCVRNA）感染的细胞中HCV繁殖的减少;而c-Fos的过表达显著增加HCV繁殖。我们进一步证实了c-Fos的积极作用，在丙型肝炎病毒的传播，通过HCV-荧光素酶报告试验和免疫荧光分析。我们发现，c-Fos水平上调HCV感染。此外，佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）诱导的c-Fos水平协同增加HCV感染。这些数据表明，c-Fos作为一个积极的调节HCV的传播，并可能有助于HCV相关的发病机制。(C)2011年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Hepatitis C virus (HCV) RNA replication requires cellular factors as well as viral non-structural proteins (NS protein). Using small interfering RNA (siRNA) library screening, we previously identified c-Fos as a host factor involved in HCV propagation. In the present study, we demonstrated that silencing of c-Fos expression resulted in decrease of HCV propagation in cell culture grown HCV (HCVcc)-infected cells; whereas overexpression of c-Fos significantly increased HCV propagation. We further confirmed the positive role of c-Fos in HCV propagation by both HCV-luciferase reporter assay and immunofluorescence analysis. We showed that c-Fos level was upregulated by HCV infection. Furthermore, phorbol 12-myristate 13-acetate (PMA)-induced c-Fos level was synergistically increased by HCV infection. These data suggest that c-Fos acts as a positive regulator of HCV propagation and may contribute to HCV-associated pathogenesis. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.