Quantitative analysis of apoptotic and necrotic outer hair cells after exposure to different levels of continuous noise
Quantitative analysis of apoptotic and necrotic outer hair cells after exposure to different levels of continuous noise
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DOI：
10.1016/j.heares.2004.04.015
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发表时间：
2004-10-01
期刊：
影响因子：
2.8
通讯作者：
Nicotera, TM
中科院分区：
文献类型：
--
作者：
Yang, WP;Henderson, D;Nicotera, TM
我们报道称，噪音暴露后 2 天，耳蜗病变的大小因外毛细胞 (OHC) 因凋亡或坏死而死亡而扩大 [听。资源。 166（2002）62]。目前的研究旨在比较两种细胞死亡途径的患病率作为暴露于不同水平的噪音后时间的函数。龙猫暴露在 104 或 108 dB SPL 的窄带噪声中 1 小时。在噪声暴露后的三个时间点（1、4 和 30 天），结合 TUNEL、caspase-3 和碘化丙啶标记来识别和记录耳蜗中缺失、凋亡和坏死 CHC 的数量。暴露于 108 dB 噪声的受试者在噪声暴露后第 1 天和第 4 天检查的耳蜗中，凋亡的 OHC 明显多于坏死的 OHC。到第 30 天，细胞凋亡和坏死病理仍在继续，尽管数量很少，但两种类型的细胞死亡之间的数量没有显着差异。暴露于 104 dB 噪声的受试者在噪声暴露后第一天的凋亡和坏死 OHC 数量出现显着差异，而在噪声暴露后第 4 天和第 30 天，这种差异在统计学上变得不显着。研究结果表明，耳蜗病变的早期扩展主要归因于细胞凋亡，而病变扩展的后期可能是细胞凋亡和坏死共同作用的结果。 (C) 2004 Elsevier B.V. 保留所有权利。
We have reported that by 2 days after noise exposure the size of cochlear lesion was expanding by outer hair cells (OHCs) dying either by apoptosis or necrosis [Hear. Res. 166 (2002) 62]. The current study was designed to compare the prevalence of the two cell death pathways as a function of time after exposure to noises of different levels. Chinchillas were exposed to a narrow band noise at either 104 or 108 dB SPL for I h. At three time points (1, 4 and 30 days) after the noise exposure, the numbers of missing, apoptotic and necrotic CHCs in the cochleas were identified and documented with a combination of TUNEL, caspase-3 and propidium iodide labeling. The subjects exposed to the 108-dB noise showed significantly more apoptotic OHCs than necrotic OHCs in the cochleas examined at days 1 and 4 after the noise exposure. By day 30, apoptotic and necrotic pathologies continued, although in small quantity, with no significant difference in quantity between two types of cell death. The subjects exposed to the 104-dB noise showed a significant difference in the numbers of apoptotic and necrotic OHCs at day I after the noise exposure, whereas the difference became statistically insignificant at day 4 and day 30 after the noise exposure. The results of the study indicate that the early expansion of cochlear lesion is attributed primarily to apoptosis, whereas the later stage of lesion expansion is likely the result of an equal contribution from apoptosis and necrosis. (C) 2004 Elsevier B.V. All rights reserved.