A SINGLE BASE SUBSTITUTION IN 16S RIBOSOMAL-RNA SUPPRESSES STREPTOMYCIN DEPENDENCE AND INCREASES THE FREQUENCY OF TRANSLATIONAL ERRORS
A SINGLE BASE SUBSTITUTION IN 16S RIBOSOMAL-RNA SUPPRESSES STREPTOMYCIN DEPENDENCE AND INCREASES THE FREQUENCY OF TRANSLATIONAL ERRORS
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DOI：
10.1016/0092-8674(91)90146-p
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发表时间：
1991-07-12
期刊：
影响因子：
64.5
通讯作者：
NOLLER, HF
中科院分区：
文献类型：
--
作者：
ALLEN, PN;NOLLER, HF
大肠杆菌16S核糖体RNA（rRNA）1469位C → U的取代抑制了链霉素依赖性，并导致翻译错误频率增加。当用16S rRNA中含有U1469突变的质粒转化时，含有rpsL252或StrM287链霉素依赖性等位基因的菌株能够在不存在链霉素的情况下生长。含有野生型蛋白质和U1469突变体16S rRNA的核糖体在体外以升高的水平错误掺入亮氨酸，与一些典型的S4 ram核糖体的水平相当。这些结果为16S rRNA参与维持翻译准确性提供了额外的支持。
A C to U substitution at position 1469 of 16S ribosomal RNA (rRNA) from Escherichia coli suppresses streptomycin dependence and causes increased translational error frequencies. Strains containing the rpsL252 or StrM287 streptomycin-dependent alleles are able to grow in the absence of streptomycin when transformed with plasmids containing the U1469 mutation in 16S rRNA. Ribosomes containing wild-type proteins and U1469 mutant 16S rRNA misincorporate leucine in vitro at elevated levels, comparable to that of some typical S4 ram ribosomes. These results provide additional support for the participation of 16S rRNA in maintaining translational accuracy.