Glycosylation of the OMP85 homolog of Porphyromonas gingivalis and its involvement in biofilm formation
Glycosylation of the OMP85 homolog of Porphyromonas gingivalis and its involvement in biofilm formation
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DOI：
10.1016/j.bbrc.2007.11.035
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发表时间：
2008-01-25
影响因子：
3.1
通讯作者：
Senpuku, Hidenobu
中科院分区：
文献类型：
--
作者：
Nakao, Ryoma;Tashiro, Yosuke;Senpuku, Hidenobu
OMP 85是所有革兰氏阴性菌中高度保守的外膜蛋白。我们研究了牙龈卟啉单胞菌，形成龈下菌斑生物膜的主要牙周病原体的一个未知的OMP 85同系物。使用对牙龈卟啉单胞菌的OMP 85特异性的外环肽抗体，loop-3 Ab，我们发现牙龈卟啉单胞菌野生型和同基因galE突变体（去糖基化菌株）之间OMP 85在SDS-PAGE凝胶上的迁移率存在差异，表明OMP 85天然以糖基化形式存在。这也得到了化学去糖基化处理后OMP 85 PAGE迁移率变化的支持。此外，loop-3 Ab与galE突变体的交叉反应强于野生型菌株;并且可以比在野生型菌株中更多地抑制galE突变体中的生物膜形成。总之，这是第一份提供OMP 85糖基化和OMP 85参与生物膜形成证据的报告。(C)2007年爱思唯尔公司All rights reserved.
OMP85 is a highly conserved outer membrane protein in all Gram-negative bacteria. We studied an uncharacterized OMP85 homolog of Porphyromonas gingivalis, a primary periodontal pathogen forming subgingival plaque biofilms. Using an outer-loop peptide antibody specific for the OMP85 of P. gingivalis, loop-3 Ab, we found a difference in the mobility of OMP85 on SDS-PAGE gel between the P. gingivalis wild-type and the isogenic galE mutant, a deglycosylated strain, suggesting that OMP85 naturally exists in a glycosylated form. This was also supported by a shift in OMP85 PAGE mobility after chemical deglycosylation treatment. Further, loop-3 Ab cross-reacted with the galE mutant stronger than the wild-type strain; and could inhibit biofilm formation in the galE mutant more than in the wildtype strain. In conclusion, this is the first report providing the evidence of OMP85 glycosylation and the involvement of OMP85 in biofilm formation. (C) 2007 Elsevier Inc. All rights reserved.