Extracellular Matrix Induces Periodontal Ligament Reconstruction In Vivo
Extracellular Matrix Induces Periodontal Ligament Reconstruction In Vivo
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DOI：
10.3390/ijms20133277
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发表时间：
2019-07-01
影响因子：
5.6
通讯作者：
Kishida, Akio
中科院分区：
文献类型：
--
作者：
Nakamura, Naoko;Ito, Ai;Kishida, Akio
牙种植治疗中的一个问题是缺乏牙周膜(PDL)，它支持牙齿，防止感染，并传递感觉，如咀嚼。本研究的目的是开发一种用于支持假牙的脱细胞PDL。为此，我们采用高静水压、脱氧核糖核酸酶和去污剂处理的方法制备了含PDL基质的小鼠脱细胞下颌骨，并对其体内重建进行了评价。拔牙后，将脱细胞的含PDL基质的下颌骨植入大鼠肾被膜下，观察到宿主细胞向基质内迁移并沿PDL胶原纤维定向生长。将摘除的脱细胞牙和脱钙牙及再钙化牙作为假牙模型，重新植入大鼠脱细胞下颌骨内，种植于肾被膜下。重建的脱细胞牙的PDL矩阵与未拔牙的脱细胞下颌骨相似。这表明脱细胞的PDL基质可以通过控制宿主细胞的迁移来重建PDL组织，这可能是一种新的牙周治疗方法。
One of the problems in dental implant treatment is the lack of periodontal ligament (PDL), which supports teeth, prevents infection, and transduces sensations such as chewiness. The objective of the present study was to develop a decellularized PDL for supporting an artificial tooth. To this end, we prepared mouse decellularized mandible bone with a PDL matrix by high hydrostatic pressure and DNase and detergent treatments and evaluated its reconstruction in vivo. After tooth extraction, the decellularized mandible bone with PDL matrix was implanted under the subrenal capsule in rat and observed that host cells migrated into the matrix and oriented along the PDL collagen fibers. The extracted decellularized tooth and de- and re-calcified teeth, which was used as an artificial tooth model, were re-inserted into the decellularized mandible bone and implanted under the subrenal capsule in rat. The reconstructed PDL matrix for the extracted decellularized tooth resembled the decellularized mandible bone without tooth extraction. This demonstrates that decellularized PDL matrix can reconstruct PDL tissue by controlling host cell migration, which could serve as a novel periodontal treatment approach.