Gamma-glutamyl transfer reactions in bacteria.
Gamma-glutamyl transfer reactions in bacteria.
复制标题
细菌中的γ-谷氨酰转移反应。
DOI：
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发表时间：
1965
期刊：
影响因子：
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通讯作者：
N. Grossowicz
中科院分区：
文献类型：
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作者：
R. Milbauer;N. Grossowicz
总结：发现γ-谷氨酰转移活性广泛分布于不同的细菌物种中。用无细胞的变形杆菌研究了γ-谷氨酰从谷胱甘肽向水和非水受体的转移。在不添加受体的情况下，γ-谷氨酰残基主要转移至水中;然而，检测到一些转移至底物，导致γ-谷氨酰谷胱甘肽形成。在存在受体（氨基酸或肽）的情况下，所有γ-谷氨酰残基均转移至添加的受体。对不同的反应产物进行了分离和鉴定。研究了γ-谷氨酰转移反应的动力学和性质。
SUMMARY: γ-Glutamyl transfer activity was found to be widely distributed in different bacterial species. The γ-glutamyl transfer from glutathione to water and acceptors other than water was studied with cell-free preparations of Proteus morganii. In the absence of added acceptor, the γ-glutamyl residue was predominantly transferred to water; however, some transfer to the substrate, resulting in the formation of γ-glutamylglutathione, was detected. In the presence of acceptors (amino acids or peptides) all the γ-glutamyl residue was transferred to the added acceptor. The different reactionproducts were isolated and identified. Kinetics and properties of the γ-glutamyl transfer reaction were studied.