Comparison of the BBL CHROMagar Staph aureus Agar Medium to Conventional Media for Detection of Staphylococcus aureus in Respiratory Samples
Comparison of the BBL CHROMagar Staph aureus Agar Medium to Conventional Media for Detection of Staphylococcus aureus in Respiratory Samples
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BBL CHROMagar 金黄色葡萄球菌琼脂培养基与传统培养基检测呼吸道样本中金黄色葡萄球菌的比较
DOI：
10.1128/jcm.42.8.3566-3569.2004
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发表时间：
2004
影响因子：
9.4
通讯作者：
K. Carroll
中科院分区：
文献类型：
--
作者：
D. Flayhart;Clara A Lema;Anita P. Borek;K. Carroll
金黄色葡萄球菌的筛查在某些患者人群中已成为常规。本研究是BBL CHROMagar金黄色葡萄球菌琼脂（CSA）培养基（BD Diagnostics，Sparks，MD）的首次临床评价。检测S.金黄色葡萄球菌在鼻监测培养和囊性纤维化（CF）患者的呼吸道样本。S.金黄色菌落在CSA上呈现淡紫色。其他微生物被抑制或产生明显不同的菌落颜色。S.通过玻片凝固酶、外源DNA酶和甘露醇发酵测定从所有培养基中鉴定金黄色葡萄球菌。采用琼脂稀释法进行药敏试验。共评价了679件样品。将所有样品接种到CSA上。将鼻监测培养物接种到羊血琼脂（SBA）（BD Diagnostics）上，并将CF患者的样本接种到甘露醇盐琼脂（MSA）（BD Diagnostics）上。在679个培养样品中，200个微生物在CSA上产生淡紫色（怀疑为S。金黄色葡萄球菌阳性180例。金黄色葡萄球菌对SBA或MSA。在200份CSA阳性样本中，191份被鉴定为S。金黄色。9个淡紫色菌落为玻片凝固酶阴性，随后鉴定为路邓葡萄球菌（1个）、表皮葡萄球菌（3个）、溶血葡萄球菌（1个）和棒状杆菌属（4个）。CSA提高了对S.金黄色葡萄球菌12株。金黄色葡萄球菌分离物被常规培养基遗漏。在192 S。回收的金黄色葡萄球菌分离株中，122株为甲氧西林敏感株，70株为甲氧西林耐药株。总体而言，CSA在本研究中的敏感性和特异性分别为99.5%和98%。在玻片凝固酶试验或对S进行的药敏试验的性能方面没有差异。金黄色葡萄球菌从CSA中的回收率与SBA或MSA相比。我们的数据支持使用CSA代替标准培养基检测S。严重污染的呼吸道样本中的金黄色葡萄球菌。
ABSTRACT Screening for Staphylococcus aureus has become routine in certain patient populations. This study is the first clinical evaluation of the BBL CHROMagar Staph aureus agar (CSA) medium (BD Diagnostics, Sparks, Md.) for detection of S. aureus in nasal surveillance cultures and in respiratory samples from cystic fibrosis (CF) patients. S. aureus colonies appear mauve on CSA. Other organisms are inhibited or produce a distinctly different colony color. S. aureus was identified from all media by slide coagulase, exogenous DNase, and mannitol fermentation assays. Susceptibility testing was performed using the agar dilution method. A total of 679 samples were evaluated. All samples were inoculated onto CSA. Nasal surveillance cultures were inoculated onto sheep blood agar (SBA) (BD Diagnostics), and samples from CF patients were inoculated onto mannitol salt agar (MSA) (BD Diagnostics). Of the 679 samples cultured, 200 organisms produced a mauve color on CSA (suspicious for S. aureus) and 180 were positive for S. aureus on SBA or MSA. Of 200 CSA-positive samples 191 were identified as S. aureus. Nine mauve colonies were slide coagulase negative and were subsequently identified as Staphylococcus lugdunensis (one), Staphylococcus epidermidis (three), Staphylococcus haemolyticus (one), and Corynebacterium species (four). CSA improved the ability to detect S. aureus by recovering 12 S. aureus isolates missed by conventional media. Of the 192 S. aureus isolates recovered, 122 were methicillin susceptible and 70 were methicillin resistant. Overall, the sensitivity and specificity of CSA in this study were 99.5 and 98%, respectively. There was no difference in the performance of the slide coagulase test or in susceptibility testing performed on S. aureus recovered from CSA compared to SBA or MSA. Our data support the use of CSA in place of standard culture media for detection of S. aureus in heavily contaminated respiratory samples.
耐甲氧西林金黄色葡萄球菌引起的医院感染的流行病学。
影响因子：
39.2
作者：
Thompson,RL;Cabezudo,I;Wenzel,RP
通讯作者：
Wenzel,RP