Binding of bullous pemphigoid antibodies to basal cells.
Binding of bullous pemphigoid antibodies to basal cells.
复制标题
大疱性类天疱疮抗体与基底细胞的结合。
DOI：
10.1111/1523-1747.ep12544466
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
H. Hatano
中科院分区：
文献类型：
--
作者：
T. Nishikawa;S. Kurihara;T. Harada;H. Hatano
12例具有基底膜区抗体的大疱性类天疱疮患者的血清样品中有8例不仅与基底膜区结合，而且与基底细胞膜和/或细胞质结合，如补体免疫荧光所观察到的。荧光素标记的寻常型天疱疮γ-球蛋白的反应，主要结合到表皮的较低细胞间隙，通过预先孵育对基底细胞具有反应性的高滴度大疱性类天疱疮血清而大大降低，而荧光素标记的落叶型天疱疮γ-球蛋白的反应主要结合到上部和中部细胞间隙，不受这些大疱性类天疱疮血清的预先应用的影响。这些结果表明，大疱性类天疱疮抗体是异质性的，可分为2种类型，并且在天疱疮抗体和对基底细胞具有反应性的大疱性类天疱疮抗体之间存在一些交叉反应。
Eight out of 12 serum samples from patients with bullous pemphigoid having basement membrane zone antibodies gave positive binding not only to the basement membrane zone but also to the basal cell membrane and/or cytoplasm as observed by complement immunofluorescence. Reaction of fluorescein labeled pemphigus vulgaris gamma-globulins, binding mainly to the lower intercellular spaces of the epidermis, was greatly reduced by the prior incubation of high-titered bullous pemphigoid sera having the reactivity to the basal cells, while that of fluorescein labeled pemphigus foliaceus gamma-globulins binding mainly to the upper and middle intercellular spaces, was not influenced by the prior application of these bullous pemphigoid sera. These results indicate that bullous pemphigoid antibodies are heterogenous and can be classified into 2 types and that some cross reaction is present between pemphigus antibodies and bullous pemphigoid antibodies having the reactivity to the basal cells.