Interaction between human cytomegalovirus UL136 protein and ATP1B1 protein
Interaction between human cytomegalovirus UL136 protein and ATP1B1 protein
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人巨细胞病毒UL136蛋白与ATP1B1蛋白之间的相互作用
DOI：
10.1590/s0100-879x2011007500144
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发表时间：
2011-12-01
影响因子：
2.3
通讯作者：
Ruan, Qiang
中科院分区：
文献类型：
--
作者：
Cui, Xin;Sun, Zheng-Rong;Ruan, Qiang
宿主和人巨细胞病毒（HCMV）之间的相互作用在感染的结果中起着关键作用。HCMV Toledo株和低传代临床分离株中存在的一个区域（称为UL/b '）含有19个额外的基因，这些基因在高传代实验室菌株AD 169中不存在。UL/B'基因产物可决定HCMV感染的体内表现。然而，对与UL/B'蛋白相互作用的宿主因子知之甚少。本研究旨在探讨HCMV UL 136蛋白的功能。通过酵母双杂交筛选，发现宿主细胞Na+/K+-ATP酶β 1亚基（ATP 1B 1）是与HCMV UL 136蛋白相互作用的候选蛋白。通过下拉测定法在体外和通过免疫荧光共定位在体内进一步评估相互作用。结果表明，UL 136蛋白在体外可与ATP 1B 1相互作用。UL 136-EGFP和ATP 1B 1-DsRed在细胞膜中的共定位表明ATP 1B 1是UL 136蛋白的伴侣。可以提出，HCMV UL 136蛋白可能在诸如细胞间传播的过程中以及在HCMV感染期间维持细胞渗透压和细胞内离子稳态中具有重要作用。
Interplay between the host and human cytomegalovirus (HCMV) has a pivotal role in the outcome of infection. A region (referred to as UL/b') present in the Toledo strain of HCMV and low passage clinical isolates contains 19 additional genes, which are absent in the highly passaged laboratory strain AD169. Products of the UL/b' genes may determine the manifestations of HCMV infection in vivo. However, little is known about the host factors, which interact with UL/b' proteins. This study was conducted to investigate the function of the HCMV UL136 protein. By yeast two-hybrid screening, the beta 1 subunit of the host Na+/K+-ATPase (ATP1B1) was identified to be a candidate protein, which interacts with the HCMV UL136 protein. The interaction was further evaluated both in vitro by pull-down assay and in vivo by immunofluorescent co-localization. The results showed that the UL136 protein can interact with ATP1B1 in vitro. Co-localization of UL136-EGFP and ATP1B1-DsRed in cell membranes suggests that ATP1B1 was a partner of the UL136 protein. It can be proposed that the HCMV UL136 protein may have important roles in processes such as cell-to-cell spread, and in maintaining cell osmotic pressure and intracellular ion homeostasis during HCMV infection.