TRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL REGULATION OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE GENE-EXPRESSION IN LIGHT-GROWN AND DARK-GROWN AMARANTH COTYLEDONS
TRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL REGULATION OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE GENE-EXPRESSION IN LIGHT-GROWN AND DARK-GROWN AMARANTH COTYLEDONS
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DOI：
10.1128/mcb.5.9.2238
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发表时间：
1985-01-01
影响因子：
5.3
通讯作者：
KLESSIG, DF
中科院分区：
文献类型：
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作者：
BERRY, JO;NIKOLAU, BJ;KLESSIG, DF
在1 - 8天龄，黑暗生长（黄化）和光生长的苋菜子叶的核酮糖1，5-二磷酸羧化酶（RuBPCase）的大亚基（LSU）和小亚基（SSU）的基因编码的表达调控进行了研究。RuBPCase比活性在光生长的子叶增加，在这8天期间的水平比黑暗生长的子叶高15倍。在两种生长条件下，LSU和SSU多肽的积累不协调。 最初检测的SSU发生后1和2天的LSU在光和暗生长的子叶，分别出现。此外，虽然LSU的水平在光照和黑暗生长的幼苗中是相似的，但SSU的量清楚地跟随酶活性的变化。这两种多肽的合成在黄化与光照生长的子叶中显着不同。在光下，在第2天首次观察到两种亚基的合成，并在子叶的整个生长过程中持续。在黑暗中，这两个亚基的合成速率远低于光，只发生在种植后第2和第5天之间的爆发。然而，这两种亚基的mRNA在第4天至第7天以相似的水平存在于黄化的子叶中（通过北方分析），并且在体外具有功能，尽管它们在第5天后在体内明显无活性。此外，由于LSU和SSU的mRNA水平都较低，在黑暗中比在光生长的幼苗，我们的研究结果表明，转录和转录后控制调节RuBPCase生产在发展中的苋菜子叶。
The regulation of expression of the genes encoding the large subunit (LSU) and small subunit (SSU) of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was examined in 1- through 8-day-old, dark-grown (etiolated) and light-grown amaranth cotyledons. RuBPCase specific activity in light-grown cotyledons increased during this 8-day period to a level 15-fold higher than in dark-grown cotyledons. Under both grown conditions, the accumulation of the LSU and SSU polypeptides was not coordinated. Initial detection of the SSU occurred 1 and 2 days after the appearance of the LSU in light- and dark-grown cotyledons, respectively. Furthermore, although the levels of the LSU were similar in both light- and dark-grown seedlings, the amount of the SSU followed clearly the changes in enzyme activity. Synthesis of these two polypeptides was dramatically different in etiolated versus light-grown cotyledons. In light the synthesis of both subunits was first observed on day 2 and continued throughout the growth of the cotyledons. In darkness the rate of synthesis of both subunits was much lower than the light and occurred only as a burst between days 2 and 5 after planting. However, mRNAs for both subunits were present in etiolated cotyledons at similar levels on days 4 through 7 (by Northern analysis) and were functional in vitro, despite their apparent inactivity in vivo after day 5. In addition, since both LSU and SSU mRNA levels were lower in dark- than in light-grown seedlings, our results indicate that both transcriptional and post-transcriptional controls modulate RuBPCase production in developing amaranth cotyledons.