Antibodies to hnRNP core proteins inhibit in vitro splicing of human beta-globin pre-mRNA
Antibodies to hnRNP core proteins inhibit in vitro splicing of human beta-globin pre-mRNA
复制标题
hnRNP 核心蛋白抗体抑制人 β-珠蛋白前体 mRNA 的体外剪接
DOI：
--
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
R. Kole
中科院分区：
文献类型：
--
作者：
H. Sierakowska;W. Szer;P. Furdon;R. Kole
人β-珠蛋白前体mRNA的体外剪接可以通过在加入前体mRNA之前用针对hnRNP核心蛋白的多克隆抗体处理剪接提取物来完全抑制。抑制剪接途径中的第一步（在5'剪接位点处的切割和外显子形成）比抑制第二步（在3'剪接位点处的切割和外显子连接）需要更多的抗体。抗hnRNP抗体还可以抑制活性核蛋白剪接复合物形成后的剪接反应，已知这发生在初始滞后期。因此，hnRNP核心蛋白似乎存在于执行前mRNA剪接的复合物中。
In vitro splicing of human beta-globin pre-mRNA can be fully inhibited by treatment of the splicing extract with polyclonal antibodies against hnRNP core proteins prior to the addition of pre-mRNA. Inhibition of the first step in the splicing pathway, cleavage at the 5' splice site and lariat formation, requires more antibodies than inhibition of the second step, cleavage at the 3' splice site and exon ligation. The anti-hnRNP antibodies can also inhibit the splicing reaction after the formation of the active nucleoprotein splicing complex which is known to occur during the initial lag period. Thus, hnRNP core proteins appear to be present in the complex that performs pre-mRNA splicing.