Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of gentically modified crops. 3. Assessing unintended effects
Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of gentically modified crops. 3. Assessing unintended effects
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DOI：
10.1021/jf052358q
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发表时间：
2006-03-22
影响因子：
6.1
通讯作者：
Jany, KD
中科院分区：
文献类型：
--
作者：
Ruebelt, MC;Lipp, M;Jany, KD
目前评估现代生物技术食品和饲料安全性的程序包括调查可能的非预期影响。为了提高检测非预期效应的概率，目前正在测试蛋白质组学等分析技术，作为现有安全性评估的补充分析工具。采用优化的双向凝胶电泳（2DE）方法研究基因工程对蛋白质组的插入效应和多效性效应。对12个转基因拟南芥株系进行了双向电泳分析，并将其种子蛋白质组与其亲本株系以及12个拟南芥生态型株系进行了比较。使用三种不同的基因和三种不同的启动子对拟南芥品系进行遗传修饰，并没有对所分析的种子蛋白质组造成意想不到的变化。转基因和非转基因株系之间的斑点数量的差异落在12个拟南芥生态型株系中发现的值的范围内，或者与引入的基因有关。
The current procedures to assess the safety of food and feed derived from modern biotechnology include the investigation of possible unintended effects. To improve the probability of detecting unintended effects, profiling techniques such as proteomics are currently tested as complementary analytical tools to the existing safety assessment. An optimized two-dimensional gel electrophoresis (2DE) method was used as a proteomics approach to investigate insertional and pleiotropic effects on the proteome due to genetic engineering. Twelve transgenic Arabidopsis thaliana lines were analyzed by 2DE, and their seed proteomes were compared to that of their parental line as well as to 12 Arabidopsis ecotype lines. The genetic modification of the Arabidopsis lines, using three different genes and three different promoters, did not cause unintended changes to the analyzed seed proteome. Differences in spot quantity between transgenic and nontransgenic lines fell in the range of values found in the 12 Arabidopsis ecotype lines or were related to the introduced gene.