THE ACTIVITY IN THE CHICKEN ALIMENTARY-TRACT OF BACTERIOPHAGES LYTIC FOR SALMONELLA-TYPHIMURIUM
THE ACTIVITY IN THE CHICKEN ALIMENTARY-TRACT OF BACTERIOPHAGES LYTIC FOR SALMONELLA-TYPHIMURIUM
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DOI：
10.1016/0923-2508(91)90187-f
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发表时间：
1991-06-01
影响因子：
2.6
通讯作者：
BARROW, PA
中科院分区：
文献类型：
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作者：
BERCHIERI, A;LOVELL, MA;BARROW, PA
从实验感染鼠伤寒沙门氏菌的鸡中分离出大量裂解鼠伤寒沙门氏菌的噬菌体。鼠伤寒沙门氏菌，而鸡饲料中的数量要少得多。溶胞菌也经常从人类的下水道系统中分离出来。其中一个是用来做三明治的。鼠伤寒感染的两日龄鸡口服和通过饲料。噬菌体在盲肠中建立的时间比沙门氏菌长，当盲肠中的沙门氏菌感染后，噬菌体消失了。鼠伤寒杆菌计数降至10（6）CFU/ml。在这些鸡的血清中没有检测到针对噬菌体的中和抗体。在第二个实验中，30只鸡中有5只同样感染了沙门氏菌。用噬菌体接种鼠伤寒沙门氏菌。在3天内，从72%的“接触”鸟类中分离出噬菌体。第二种噬菌体是从污水中分离的，与三种沙门氏菌同时接种于初孵雏鸡。鼠伤寒沙门氏菌，产生了相当大的减少死亡率的鸟类。这种效应仅通过接种高浓度的噬菌体（> 10（10）PFU/ml）产生。噬菌体使S.接种后12 h内，作物、小肠和盲肠中的鼠伤寒杆菌数量减少，在感染后24和48 h，肝脏中的细菌数量减少较少。
Bacteriophages lytic for Salmonella typhimurium were isolated in considerable numbers from chickens experimentally infected with S. typhimurium, and in much lower numbers from the chicken feed. Lytic phages were also regularly isolated from human sewerage systems. One of these was used to inoculate S. typhimurium - infected two day-old chickens orally and via the feed. The phage took longer to establish in the caeca than did the Salmonella and it disappeared when the caecal S. typhimurium counts fell to 10(6) CFU/ml. No neutralizing antibodies to the phage were detected in the serum of these chickens. In a second experiment, five of 30 chickens similarly infected with S. typhimurium were inoculated with the phage. Within 3 days, the phage was isolated from 72% of the "in-contact" birds. A second phage, isolated from sewage, when inoculated into newly-hatched chickens simultaneously with any of 3 strains of S. typhimurium, produced a considerable reduction in mortality in the birds. This effect was only produced by inoculation of high concentrations of phage (> 10(10) PFU/ml). The phage produced reductions in the viable numbers of S. typhimurium in the crop, small intestine and caeca for up to 12 h after inoculation, with smaller reductions in bacterial numbers in the liver at 24 and 48 h after infection.