The Escherichia coli SMC Complex, MukBEF, Shapes Nucleoid Organization Independently of DNA Replication
The Escherichia coli SMC Complex, MukBEF, Shapes Nucleoid Organization Independently of DNA Replication
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DOI：
10.1128/jb.00957-12
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发表时间：
2012-09-01
影响因子：
3.2
通讯作者：
Sherratt, David
中科院分区：
文献类型：
--
作者：
Badrinarayanan, Anjana;Lesterlin, Christian;Sherratt, David
SMC（染色体结构维持）复合体在组织和维持染色体中普遍发挥作用。大肠杆菌SMC复合物的功能性荧光衍生物MukBEF形成与复制起始区（上）相关的病灶。MukBEF损伤导致稳态细胞中on和其他基因座的错误定位。这些观察导致了一个早期的建议，MukBEF位置新复制的姐妹口。我们在这里表明，MukBEF产生和维持独立于DNA复制的染色体位点的细胞定位。通过在不存在DNA复制的情况下耗尽Muk组分而快速损害MukBEF功能导致MukBEF焦点的损失以及on和其他基因座的错误定位，而快速Muk合成导致快速MukBEF焦点形成，但正常染色体基因座定位的恢复缓慢。
SMC (structural maintenance of chromosomes) complexes function ubiquitously in organizing and maintaining chromosomes. Functional fluorescent derivatives of the Escherichia coli SMC complex, MukBEF, form foci that associate with the replication origin region (on). MukBEF impairment results in mispositioning of on and other loci in steady-state cells. These observations led to an earlier proposal that MukBEF positions new replicated sister oris. We show here that MukBEF generates and maintains the cellular positioning of chromosome loci independently of DNA replication. Rapid impairment of MukBEF function by depleting a Muk component in the absence of DNA replication leads to loss of MukBEF foci as well as mispositioning of on and other loci, while rapid Muk synthesis leads to rapid MukBEF focus formation but slow restoration of normal chromosomal locus positioning.