Polarized light retardation analysis allows for the evaluation of tension in individual stress fibers
Polarized light retardation analysis allows for the evaluation of tension in individual stress fibers
复制标题
偏振光延迟分析可以评估单个应力纤维的张力
DOI：
10.1016/j.bbrc.2022.06.066
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发表时间：
2022
影响因子：
3.1
通讯作者：
Nakamura Masanori
中科院分区：
文献类型：
--
作者：
Sugita Shukei;Hozaki Masatoshi;Matsui Tsubasa S.;Nagayama Kazuaki;Deguchi Shinji;Nakamura Masanori
细胞应力纤维的张力在决定细胞迁移、形态形成和蛋白质合成等生物学过程中起着关键作用。我们以前的研究开发了一种方法来评估基于偏振光的光弹性相关延迟的SF中产生的细胞收缩力;然而，我们采用了活细胞，这可能导致延迟而不是收缩力的增加。因此，本研究旨在证实偏振光延迟由于收缩而固有地增加，而与细胞活性无关。我们还探讨了延迟增加SF收缩的原因。我们使用从血管平滑肌细胞物理分离的SF来停止细胞活性。测量ATP给药后SF的阻滞，负责收缩SF。在光学显微镜和电子显微镜下对SF进行成像，以测量SF的长度、宽度和延迟。ATP处理后，分离的SFs的延迟明显高于处理前。因此，我们证实，延迟增加与个别SF的张力升高。此外，SF直径减小，而SF长度几乎保持不变。因此，我们得出结论，收缩力驱动的密度增加的SF是偏振光延迟上升的主要因素。
The tension in the stress fibers (SFs) of cells plays a pivotal role in determining biological processes such as cell migration, morphological formation, and protein synthesis. Our previous research developed a method to evaluate the cellular contraction force generated in SFs based on photoelasticity-associated retardation of polarized light; however, we employed live cells, which could have caused an increase in retardation and not contraction force. Therefore, the present study aimed to confirm that polarized light retardation increases inherently due to contraction, regardless of cell activity. We also explored the reason why retardation increased with SF contractions. We used SFs physically isolated from vascular smooth muscle cells to stop cell activity. The retardation of SFs was measured after ATP administration, responsible for contracting SFs. The SFs were imaged under optical and electron microscopes to measure SF length, width, and retardation. The retardation of isolated SFs after ATP administration was significantly higher than before. Thus, we confirmed that retardation increased with elevated tension in individual SFs. Furthermore, the SF diameter decreased while the SF length remained almost constant. Thus, we conclude that a contraction force-driven increase in the density of SFs is the main factor for the rise in polarized light retardation.