Effect of germination potential on storage lipids and transcriptome changes in premature developing seeds of oilseed rape (Brassica napusL.)
Effect of germination potential on storage lipids and transcriptome changes in premature developing seeds of oilseed rape (Brassica napusL.)
复制标题
发芽势对油菜过早发育种子贮藏脂质和转录组变化的影响
DOI：
10.1007/s00122-020-03636-7
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发表时间：
2020
影响因子：
5.4
通讯作者：
Jiang Lixi
中科院分区：
文献类型：
--
作者：
Zhu Le;Zhao Xinze;Xu Ying;Wang Qian;Wang Haoyi;Wu Dezhi;Jiang Lixi
Key messageWe提供了一个中等大小的基因库，用于选择或操纵有利于获得种子休眠的候选基因，通过阻断发育中种子的脂质降解，揭示油菜种子含油量的升高，AbstractIn油菜，早熟种子的萌发潜力和种子脂质的最终水平之间的关联，以及潜在的机制，是难以捉摸的。在这里，我们调查了一系列油菜品种中早熟种子发芽率的表型差异。我们比较了深，中度和非休眠基因型之间的动态脂质积累，并比较了多对深和非休眠基因型之间授粉后40天的种子的转录组。我们确定了一个广泛的差异，早熟种子的发芽率和发芽势之间的关联和脂肪酸含量的变化在种子成熟后期。深度休眠种子和非休眠种子之间转录组的比较揭示了休眠差异的遗传基础，例如参与赤霉素和脱落酸生物合成和/或信号传导、脂肪酸代谢途径和结构的基因的不同表达水平。种子细胞壁。我们提供了一个中等大小的基因库，用于选择或操纵有利于获得种子休眠的候选基因，通过阻断发育中种子的脂质降解来提高油菜种子含油量。
Key messageWe provided a gene pool of moderate size for selecting or manipulating the candidate genes that favour the acquisition of seed dormancy, shedding light on the elevation of seed oil content in oilseed rape by blocking lipid degradation in developing seeds.AbstractIn oilseed rape, the association between the germination potential of premature seeds and the final level of seed lipids, and the underlying mechanism, is elusive. Here, we investigated phenotypic differences in the germination percentage of premature seeds in a collection of oilseed rape cultivars. We compared the dynamic lipid accumulation between the deep-, moderate- and non-dormant genotypes and compared the transcriptomes of the seeds at 40 days after pollination between multiple pairs of deep- and non-dormant genotypes. We identified a wide range of differences in germination percentage of premature seeds and the association between the germination potential and the change of fatty acid content at late stage of seed maturation. The comparisons of transcriptomes between deep- and non-dormant seeds revealed the genetic basis for the dormant difference, e.g. the different expression levels of the genes involved in gibberellic and abscisic acid biosynthesis and/or signalling, fatty acid metabolic pathways, and the structure of seed cell wall. We provided a gene pool of moderate size for selecting or manipulating the candidate genes that favour the acquisition of seed dormancy, shedding light on the elevation of seed oil content in oilseed rape by blocking lipid degradation in developing seeds.