DPATJ plays a role in retinal morphogenesis and protects against light-dependent degeneration of photoreceptor cells in the Drosophila eye
DPATJ plays a role in retinal morphogenesis and protects against light-dependent degeneration of photoreceptor cells in the Drosophila eye
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DOI：
10.1002/dvdy.20595
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发表时间：
2006-04-01
影响因子：
2.5
通讯作者：
Knust, E
中科院分区：
文献类型：
--
作者：
Richard, M;Grawe, F;Knust, E
在上皮细胞中建立顶基极性是其功能的先决条件。果蝇感光细胞源自上皮细胞，它们的顶膜在蛹发育期间经历精心的分化，形成光敏感杆和相关的柄膜。Crumbs（Crb）是一种跨膜蛋白，参与维持胚胎上皮细胞的极性，将柄定义为顶膜的一个亚结构域。Crb组织由几个含有PDZ结构域的蛋白组成的复合物，包括DPATJ（以前称为Discs lost）。利用DPATJ突变株系，其中只有一个截断形式的蛋白质的合成，我们证明，DPATJ是必要的稳定性的Crb复合物在柄膜，是至关重要的柄膜发育和横纹肌维持在蛹后期阶段。此外，DPATJ保护光诱导的感光细胞变性。
The establishment of apicobasal polarity in epithelial cells is a prerequisite for their function. Drosophila photoreceptor cells derive from epithelial cells, and their apical membranes undergo elaborate differentiation during pupal development, forming photosensitive rhabdomeres and associated stalk membranes. Crumbs (Crb), a transmembrane protein involved in the maintenance of epithelial polarity in the embryo, defines the stalk as a subdomain of the apical membrane. Crb organizes a complex composed of several PDZ domain-containing proteins, including DPATJ (formerly known as Discs lost). Taking advantage of a DPATJ mutant line in which only a truncated form of the protein is synthesized, we demonstrate that DPATJ is necessary for the stability of the Crb complex at the stalk membrane and is crucial for stalk membrane development and rhabdomere maintenance during late pupal stages. Moreover, DPATJ protects against light-induced photoreceptor degeneration.