Restriction of proliferation of primordial germ cells by the irradiation of Japanese quail embryos with soft X-rays
Restriction of proliferation of primordial germ cells by the irradiation of Japanese quail embryos with soft X-rays
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DOI：
10.1016/s1095-6433(01)00375-0
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发表时间：
2001-08-01
影响因子：
2.3
通讯作者：
Ono, T
中科院分区：
文献类型：
--
作者：
Li, HC;Kagami, H;Ono, T
原始生殖细胞是配子的祖细胞。禽类原生殖细胞位于胚胎期透明质区的中部。在进一步孵化后不久，它们迁移到胚胎外的生发新月，然后一旦血管形成，它们就进入循环，最终定居在性腺原基。我们开发了一种使用软X射线照射(功率18千伏，距离20厘米)来减少日本鹌鹑胚胎中PGC数量的简单方法，这将有助于为PGC移植研究准备受体胚胎。胚胎在孵化前对40枚S进行软X射线照射，孵育2天后循环中PGCs的浓度不到对照组的1/5。与在孵化前或孵化第2天暴露的对照组相比，孵化第6天的胚胎含有大约一半的PGC数量。考虑到对原生殖细胞增殖的抑制以及存活率和孵化率，建议对40只S进行照射。(C)2001 Elsevier Science Inc.保留所有权利。
Primordial germ cells (PGCs) are the progenitor cells for the gametes. Avian PGCs are located in the central region of the area pellucida at the blastoderm stage. Shortly after further incubation, they migrate to the extra-embryonic germinal crescent, and then as soon as the blood vessels form, they enter the circulation and finally settle in the gonadal primordium. We have developed a simple method using soft X-ray irradiation (18 kV power, 20 cm distance) to reduce the number of PGCs in Japanese quail embryos, which should be useful in preparing recipient embryos for PGC-transfer studies. When embryos were exposed to the soft X-rays for 40 s before incubation, the concentration of circulating PGCs was less than one-fifth that in controls after 2 days of incubation. Embryos at day 6 of incubation contained approximately half the number of PGCs compared to controls when they were exposed before or at day 2 of incubation. Irradiation for 40 s is recommended taking into consideration the restriction of proliferation of PGCs, and viability and hatchability. (C) 2001 Elsevier Science Inc. All rights reserved.