Localization of BRRN1, the human homologue of Drosophila barr, to 2q11.2.
Localization of BRRN1, the human homologue of Drosophila barr, to 2q11.2.
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DOI：
10.1006/geno.1997.5021
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发表时间：
1997-12
期刊：
影响因子：
4.4
通讯作者：
O. A. Cabello;A. Baldini;M. Bhat;H. Bellen;J. Belmont
中科院分区：
文献类型：
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作者：
O. A. Cabello;A. Baldini;M. Bhat;H. Bellen;J. Belmont
cDNA探针证实BRRN 1定位于人类2号染色体BRRN 1的定位（图1A）（14）。为了鉴定含有BRRN 1的YAC克隆，我们通过PCR筛选果蝇巴尔的同源物CEPH-Mark II YAC文库。我们使用了两个引物对，设计用于产生对应于位置448-625（引物组I）和2229-2402 Olga A的PCR产物。Cabello，* Antonio Baldini，* Manzoor Bhat，†（引物组II）。这两组引物Hugo Bellen，* Zhao和John W.贝尔蒙特 *§在人基因组DNA模板上产生独特的PCR扩增产物。7个PCR阳性克隆在Southern分析中也表现出BRRN 1杂交。克隆 * 霍华德休斯医学研究所分子和人类遗传学系、细胞生物学系和539 D8系包括贝勒医学院微生物学和免疫学及儿科学鉴定的所有限制性片段，基因组Southern印迹（图1A）。如前所述（1）制备Houston，Texas 77030 YAC 539 D8的RT-PCR产物，并用作FISH的探针，产生离散杂交
cDNA probe confirmed that BRRN1 maps to chromosome 2 Localization of BRRN1, the Human (Fig. 1A) (14). To identify BRRN1-containing YAC clones we screened the Homologue of Drosophila barr, CEPH-Mark II YAC library by PCR. We used two oligonucleoto 2q11.2 tide primer pairs designed to yield PCR products corresponding to positions 448–625 (primer set I) and 2229–2402 Olga A. Cabello,* Antonio Baldini,* Manzoor Bhat,† (primer set II) in the BRRN1 cDNA. Both sets of primers Hugo Bellen,*‡ and John W. Belmont*§ yielded unique PCR amplification products on human genomic DNA template. Seven of the PCR-positive clones also exhibited BRRN1 hybridization on Southern analysis. Clone *Department of Molecular and Human Genetics, †Howard Hughes Medical Institute, ‡Department of Cell Biology, and §Departments of 539D8 encompassed all restriction fragments identified by Microbiology & Immunology and Pediatrics, Baylor College of Medicine, the genomic Southern blots (Fig. 1A). Alu-PCR products of Houston, Texas 77030 YAC 539D8 were prepared as previously described (1) and used as probes for FISH, yielding a discrete hybridization