IFP35 is involved in the antiviral function of interferon by association with the viral tas transactivator of bovine foamy virus
IFP35 is involved in the antiviral function of interferon by association with the viral tas transactivator of bovine foamy virus
复制标题
IFP35 通过与牛泡沫病毒的病毒 Tas 反式激活因子结合参与干扰素的抗病毒功能
DOI：
10.1128/jvi.02249-07
复制
发表时间：
2008-05-01
影响因子：
5.4
通讯作者：
Geng, Yunqi
中科院分区：
文献类型：
--
作者：
Tan, Juan;Qiao, Wentao;Geng, Yunqi
干扰素诱导蛋白（IFPs）发挥多种功能，对应于不同的干扰素信号。然而，许多IFPs的细胞内功能尚未完全表征。在这里，我们报告，IFP 35，IFP家族的成员，分子量为35 kDa，可以与牛泡沫病毒（BFV）的牛Tas（BTas）调节蛋白相互作用。这种相互作用涉及IFP 35的NID 2（IFP 35/Nmi同源结构域）和BTas的中心结构域。IFP 35的过表达干扰BTas激活病毒基因转录并抑制病毒复制的能力。干扰RNA对内源性IFP 35的去除可以促进BFV的活化，表明IFP 35在病毒基因表达中具有抑制功能。此外，IFP 35可以与原型FV的同源调节蛋白相互作用，并阻止病毒复制和抑制病毒转录。我们的研究表明，IFP 35可能代表了一种新的干扰素介导的抗病毒活性在宿主生物体中发挥作用，在FV潜伏期的维护途径。
Interferon-induced proteins (IFPs) exert multiple functions corresponding to diverse interferon signals. However, the intracellular functions of many IFPs are not fully characterized. Here, we report that IFP35, a member of the IFP family with a molecular mass of 35 kDa, can interact with the bovine Tas (BTas) regulatory protein of bovine foamy virus (BFV). The interaction involves NID2 (IFP35/Nmi homology domain) of IFP35 and the central domain of BTas. The overexpression of IFP35 disturbs the ability of BTas to activate viral-gene transcription and inhibits viral replication. The depletion of endogenous IFP35 by interfering RNA can promote the activation of BFV, suggesting an inhibitory function of IFP35 in viral-gene expression. In addition, IFP35 can interact with the homologous regulatory protein of prototype FV and arrest viral replication and repress viral transcription. Our study suggests that IFP35 may represent a novel pathway of interferon-mediated antiviral activity in host organisms that plays a role in the maintenance of FV latency.