The humoral immune response and protective efficacy of vaccination with inactivated split and whole influenza virus vaccines in BALB/c mice
The humoral immune response and protective efficacy of vaccination with inactivated split and whole influenza virus vaccines in BALB/c mice
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DOI：
10.1016/j.vaccine.2006.05.040
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发表时间：
2006-11-10
期刊：
影响因子：
5.5
通讯作者：
Haaheim, Lars Reinhardt
中科院分区：
文献类型：
--
作者：
Cox, Rebecca Jane;Hovden, Arnt-Ove;Haaheim, Lars Reinhardt
最近，开发大流行性流感疫苗的紧迫性导致了对全病毒疫苗使用的重新评估。我们比较了小鼠的体液免疫应答和流感病毒裂解疫苗的保护效力。全病毒疫苗的免疫原性更强，特别是在第一剂疫苗接种后，通常会引起更多的全身性抗体分泌细胞，以及更早和更高的中和抗体应答。用一剂全病毒疫苗免疫更有效地减少了非致死性同源病毒攻击后的病毒脱落，但两剂裂解病毒疫苗在限制病毒复制方面最有效，这与高流感特异性血清IgG浓度相关。两种疫苗制剂诱导不同的T辅助细胞特征，特别是在一剂疫苗后;裂解病毒疫苗诱导2型偏倚应答，而全病毒疫苗引起显性I型应答。(c)2006爱思唯尔有限公司版权所有。
Recently the urgency of developing a pandemic influenza vaccine has lead to the re-evaluation of the use of whole virus vaccine. We have compared the humoral immune response and the protective efficacy of whole and split influenza virus vaccines in mice. Whole virus vaccine was more immunogenic particularly after the first dose of vaccine, generally eliciting higher numbers of systemic antibody secreting cells and an earlier and higher neutralising antibody response. Immunisation with one dose of whole virus vaccine more effectively reduced viral shedding upon non-lethal homologous viral challenge, but two doses of split virus vaccine was most effective at limiting viral replication and this was correlated with high influenza specific serum IgG concentrations. The two vaccine formulations induced different T helper profiles particularly after one dose of vaccine; split virus vaccine induced a type 2 bias response, whereas whole virus vaccine elicited a dominant type I response. (c) 2006 Elsevier Ltd. All rights reserved.