RADIOIMMUNOASSAY OF BRAIN PEPTIDES - EVALUATION OF A METHODOLOGY FOR ASSAY OF BETA-ENDORPHIN AND ENKEPHALIN
RADIOIMMUNOASSAY OF BRAIN PEPTIDES - EVALUATION OF A METHODOLOGY FOR ASSAY OF BETA-ENDORPHIN AND ENKEPHALIN
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DOI：
10.1016/0024-3205(77)90414-3
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发表时间：
1977-01-01
期刊：
影响因子：
6.1
通讯作者：
BLOOM, F
中科院分区：
文献类型：
--
作者：
ROSSIER, J;BAYON, A;BLOOM, F
大脑中β的水平。脑内啡,.alpha。用放射免疫法测定不同牺牲方法后的-内啡肽和脑啡肽含量。微波辐照的效果并不比斩首后煮沸完整组织好，后一种方法的值为。-内啡肽比斩首高10倍。与此同时，斩首之后是煮沸。-内啡肽不再检测到。在脑组织中对几种提取介质，磷酸盐缓冲盐水，5% TCA[三氯乙酸]HCl甲醇和1N HOAc[乙酸]进行评估，结果表明，最后一种提取介质对两者都最满意。-内啡肽和脑啡肽。因为度量。-内啡肽很容易被脑匀浆水解，因此出现- α。-内啡肽，组织的破坏改变了大脑中阿片肽的含量。
The brain [rat] levels of .beta.-endorphin, .alpha.-endorphin and enkephalin were measured by radioimmunoassay after different methods of sacrifice. Microwave irradiation was not better than decapitation followed by boiling of the intact tissue, the latter procedure giving values of .beta.-endorphin 10-fold higher than decapitation alone. Concurrently when decapitation was followed by boiling, .alpha.-endorphin was no longer detected. Evaluation in brain tissue of several extraction media, phosphate buffered saline, 5% TCA [trichloroacetic acid] HCl methanol, and 1N HOAc [acetic acid], showed the last to be the most satisfactory for both .beta.-endorphin and enkephalin. Since .beta.-endorphin was readily hydrolized by brain homogenates with consequent appearance of .alpha.-endorphin, disruption of tissue modifies the content of opioid peptides in brain.