Immunolocalization of CB1 receptor in rat striatal neurons:: A confocal microscopy study
Immunolocalization of CB1 receptor in rat striatal neurons:: A confocal microscopy study
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DOI：
10.1002/syn.20047
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发表时间：
2004-09-01
期刊：
影响因子：
2.3
通讯作者：
Bernardi, G
中科院分区：
文献类型：
--
作者：
Fusco, FR;Martorana, A;Bernardi, G
一些证据表明，大麻素，除其他功能外，参与运动控制。虽然大麻素受体（CB 1）的mRNA已被观察到在中型多刺神经元的纹状体，精确定位的CB 1在纹状体细胞之间的蛋白质水平的描述仍然缺乏。因此，我们进行了免疫组织化学研究，光和共聚焦显微镜，以确定表达CB 1的神经元亚群，并评估这些神经元内的受体的分布。在我们的单标记光学显微镜研究中，CB 1被观察到在大多数中等大小的神经元的尾壳核。然而，CB 1也存在于分散在整个纹状体的大型神经元中。我们的双标记研究表明，89.3%的基质投射神经元含有CB 1，而56.4%的补丁投射神经元被标记为CB 1。为了研究纹状体中间神经元的不同亚类之间是否存在CB 1，我们进行了双标记研究，匹配CB 1和每个纹状体中间神经元标记物，即胆碱乙酰转移酶，小清蛋白，钙视黄蛋白和一氧化氮合酶。我们的双标记研究表明，大多数小白蛋白免疫反应中间神经元（86.5%），超过三分之一（39.2%）的胆碱能中间神经元，约三分之一（30.4%）的NOS阳性神经元被标记为CB 1。钙视黄蛋白免疫标记的神经元缺乏CB 1。(C)2004 Wiley-Liss，Inc.
Several lines of evidence indicate that cannabinoids, among other functions, are involved in motor control. Although cannabinoid receptors (CB1) mRNA has been observed in medium-sized spiny neurons of the striatum, a description of the precise localization of CB1 at a protein level among striatal cells is still lacking. Therefore, we performed immunohistochemical studies with light and confocal microscopy to identify neuronal subpopulations that express CB1 and to assess the distribution of the receptor within these neurons. In our single label light microscopy study, CB1 was observed in most medium-sized neurons of the caudate-putamen. However, CB1 was also present in large-sized neurons scattered throughout the striatum. Our dual-label study showed that 89.3% of projection neurons in matrix contain CB1, and that 56.4% of projection neurons in patch are labeled for CB1. To investigate the presence of CB1 among the different subclasses of striatal interneurons we performed a double-labeling study matching CB1 and each of the striatal interneuron markers, namely, choline acetyl-transferase, parvalbumin, calretinin, and nitric oxide synthase. Our double-label study showed that most parvalbumin immunoreactive interneurons (86.5%), more than one-third (39.2%) of cholinergic interneurons, and about one-third (30.4%) of the NOS-positive neurons are labeled for CB1. Calretinin-immunolabeled neurons were devoid of CB1. (C) 2004 Wiley-Liss, Inc.